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We narrowed to 399 results for: CAPS;

Showing: 181 - 200 of 399 results
  1. Weighing Reagents Protocol

    Type
    Protocol
    ...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...
  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized...
  3. Five Popular Model Organisms

    Type
    Blog Post
    ...study diseases such as cancer since mice better recapitulate the complex interactions between cancer cells...studied in other model organisms that better recapitulate in vivo conditions similar to humans. For example...
  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe via biotin...probe that is cut will continue to flow and be captured by an antibody further along on the strip. Highlights...
  5. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...major equipment can be defined as equipment with capital costs greater than $5,000 but this can depend on...like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools as possible, this even includes...
  6. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...transfer increases the metabolic and metastatic capacity of cancer cells. This is certainly just the beginning...brightness, photostability, and multiplexing capabilities. bioRxiv 2024.12.16.628673. https://doi.org/...
  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...
  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...
  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...
  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... cassettes, making them advantageous for small capacity vectors like AAV. In addition to the mammalian...Yang. “Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” ...
  11. Video Library

    Type
    Protocol
    ...Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos... from an Agarose Gel Protocol How-To Videos Screencapture walk-throughs to help guide you through Addgene's...
  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...against common tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176...associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH alleles off or on enables...
  13. Using a Light Microscope Protocol

    Type
    Protocol
    ...digital cameras attached to them that allow you to capture images directly to a computer, but many microscopes... your drawing skills. Regardless of your image capture method, be sure to record the magnification used...
  14. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing... number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image...
  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...you are using a polymerase with proofreading capabilities. Note: See manufacturer’s instructions for specific... of the reaction mix. Its solvent and buffer capabilities provide a suitable environment for the DNA amplification...
  16. Western Blot

    Type
    Protocol
    ...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X...
Showing: 181 - 200 of 399 results