...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

...of drugs, and developing internal operational capabilities. In just two years as a healthcare consultant...equity research firms, biotech start-ups, venture capital firms, and more. Essentially any role in the broad...

...areas associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout...behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface. The ...

...hard to raise because they beat on each other in captivity. Sticklebacks demonstrate unusual parallel adaptation...(hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis. They used...

...integration of cargo without using screening markers. Capability to construct a strain library with different ... that same year. Her research is focused on the escape mechanism of genome editing in microorganisms which...

.... Since target specificity is determined by the capsid of the AAV, many of the vectors in Addgene’s catalog...including the retrograde AAVrg and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps...

...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized...

...study diseases such as cancer since mice better recapitulate the complex interactions between cancer cells...studied in other model organisms that better recapitulate in vivo conditions similar to humans. For example...

...with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe via biotin...probe that is cut will continue to flow and be captured by an antibody further along on the strip. Highlights...

...major equipment can be defined as equipment with capital costs greater than $5,000 but this can depend on...like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools as possible, this even includes...

...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...

...biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...

...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

...brightness, photostability, and multiplexing capabilities. bioRxiv. 2025 Jan 11:2024.12.16.628673. doi...

...pull-down assay, wherein a protein of interest can be captured along with its associated partners. Common pull-down...using antibodies against the target protein. The captured protein complexes are then analysed by mass spectrometry...

... cassettes, making them advantageous for small capacity vectors like AAV. In addition to the mammalian...Yang. “Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” ...

...Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos...standard laboratory procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's...

...against common tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176...associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH alleles off or on enables...

...digital cameras attached to them that allow you to capture images directly to a computer, but many microscopes... your drawing skills. Regardless of your image capture method, be sure to record the magnification used...