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We narrowed to 569 results for: CIT

Showing: 181 - 200 of 569 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...can now assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting...changing certain amino acids you can change the specificity,” explained Zhanar Abil of the University of ...sequence. By comparison, Abil explained, the specificity of other RNA binding domains depends on the secondary...

  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...introduction to pCri, please see the original publication cited below. pCri plasmids are available either individually...

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...fluorescent protein to create three colors. The specificity of these orthologs is key: due to differences...illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential imaging...useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References Multicolor...

  4. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... Packaged on Request viral vector prep.    Excited? So are we! This expansion will give researchers... and educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging...

  5. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...accepted that day and was excited for the start of my co-op in January. This excitement did not come without...hopeful to continue on in the process and I was excited when I received an email to invite me back to conduct...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

  7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle...chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects....done with CRISPR technology in just 2 years and excitement about how much more there is left to do with ...

  8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...cell activity, enables researchers to silence or incite neuronal firing and study subsequent effects on...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow...

  9. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics...going through the pdfs of the 26,000 publications cited. If the information seems incomplete or unclear,...line distributor. A success all around! If you’re excited about this resource and want to learn more, check...

  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...on this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training...need to move around every 3-6 years—a short and exciting ride Complicated matrix structures are common...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless of excitation wavelength... emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure of autophagy...

  12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...to the research community.    So, why are we so excited? For starters, we believe that this approach can... for a host of other AAV serotypes; it will be exciting to test this approach with different capsids. ...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging (Andrews et ...recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  Addgene will ... educational materials to the resource. We are excited to work with all of you to make this the best and...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...regions are unique to each antibody and convey specificity to the target while constant regions are identical...resulting chimeric antibody retains the binding specificity to the original target and can be used with secondary...capabilities this process is frequently used to reduce immunogenicity and thereby enhance the therapeutic potential...

  15. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein (GFP) because of...compared to using a high-intensity laser to rapidly excite GFP. As a result of the different mechanisms to...Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible Subcellular Imaging...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...production to individual labs, but now we’re very excited to provide ready-to-use CRISPR lentiviral preps...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

  17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination...model for this study (Ref 6)). In addition, it was exciting to see that the use of optogenetic tools is expanding...observe the function of immune cells that are not excitable but responsive to calcium. We’d love to expand...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...

  19. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...towards conjugates.  Storage   Once you get your exciting new antibody, the second thing you’ll want to ...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ... need to know the wavelength of laser needed to excite them - and it’s a good idea to make sure that laser...

  20. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found that YFP and... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...
Showing: 181 - 200 of 569 results