We narrowed to 575 results for: CIT

...plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch...importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used...its ever-evolving needs. For now, we have many exciting things planned in 2023, from the expansion of ...

...can now assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting...changing certain amino acids you can change the specificity,” explained Zhanar Abil of the University of ...sequence. By comparison, Abil explained, the specificity of other RNA binding domains depends on the secondary...

...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...introduction to pCri, please see the original publication cited below. pCri plasmids are available either individually...

...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... Packaged on Request viral vector prep.    Excited? So are we! This expansion will give researchers... and educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging...

...accepted that day and was excited for the start of my co-op in January. This excitement did not come without...hopeful to continue on in the process and I was excited when I received an email to invite me back to conduct...

...fluorescent protein to create three colors. The specificity of these orthologs is key: due to differences...illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential imaging...useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References Multicolor...

...reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics...going through the pdfs of the 26,000 publications cited. If the information seems incomplete or unclear,...line distributor. A success all around! If you’re excited about this resource and want to learn more, check...

...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

...cell activity, enables researchers to silence or incite neuronal firing and study subsequent effects on...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow...

...") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle...chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects....done with CRISPR technology in just 2 years and excitement about how much more there is left to do with ...

...regions are unique to each antibody and convey specificity to the target while constant regions are identical...resulting chimeric antibody retains the binding specificity to the original target and can be used with secondary...capabilities this process is frequently used to reduce immunogenicity and thereby enhance the therapeutic potential...

...on this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training...need to move around every 3-6 years—a short and exciting ride Complicated matrix structures are common...

...School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging (Andrews et ...recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  Addgene will ... educational materials to the resource. We are excited to work with all of you to make this the best and...

...dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless of excitation wavelength... emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure of autophagy...

...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...to the research community.    So, why are we so excited? For starters, we believe that this approach can... for a host of other AAV serotypes; it will be exciting to test this approach with different capsids. ...

...production to individual labs, but now we’re very excited to provide ready-to-use CRISPR lentiviral preps...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

...Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination...model for this study (Ref 6)). In addition, it was exciting to see that the use of optogenetic tools is expanding...observe the function of immune cells that are not excitable but responsive to calcium. We’d love to expand...

...towards conjugates.  Storage   Once you get your exciting new antibody, the second thing you’ll want to ...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ... need to know the wavelength of laser needed to excite them - and it’s a good idea to make sure that laser...

... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...

... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...