We narrowed to 268 results for: aav
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TypeBlog Post...Delivery Methods and A Match Made in Heaven: CRISPR and AAV Day 6: Bacteria: CRISPR Methods for Bacterial Genome...
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Plan Your Experiment
TypeCollection... screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used...used to generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible.../or gRNA Infects dividing and non-dividing cells AAV is the least toxic method for in vivo viral delivery... -
Optogenetics Plasmid Collection
TypeCollection...Collection You may also like... Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics...Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid... -
Neurodegeneration Research Collection
TypeCollection... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies...Oct 18. Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders...Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality...Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene delivery...immune responses (Fenno et al., 2011). However, rAAVs lack the ability to specifically infect defined ... promoters, on the other side, are too long for rAAVs, which can only package sequences shorter than 5...vectors in combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter... 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry... -
What the HEK?
TypeBlog Post...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells ... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors. ...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pX601 61591 Mammalian/AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus...pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S. pyogenes...pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm... none S. pyogenes Gersbach PX552 60958 Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248... -
Adeno-associated virus (AAV) Guide
TypeGuide...adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production...both key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ in...Parts of the AAV Transfer Plasmid Parts of the AAV Packaging Plasmid Producing Your rAAV AAV Serotypes and...Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production Integration AAV Types Common Uses...Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at ...Virus (AAV) for Cell and Gene Therapy Adeno-associated Viruses (AAVs) for Genome Editing Using AAV for Neuronal...More AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV Purification... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...crowdsourced Data Hub (built off of the concept of our AAV Data Hub) to share further validation data with you... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...manipulations such as injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post... of a live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in AAV Dual promoter Separate...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate...expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing... -
DAP arrays for multiplex gene editing
TypeBlog Post...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...to control the CRISPR system activity. Using an AAV vector may help with size limitations. In addition... -
The time and cost required to make a plasmid
TypeBlog Post...plasmids alone. (And we expect that resources like our AAV Data Hub will help scientists save even more!) If... -
Hot Plasmids - February 2022
TypeBlog Post...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...