Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 401 results for: caps

Showing: 181 - 200 of 401 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    .... Since target specificity is determined by the capsid of the AAV, many of the vectors in Addgene’s catalog...including the retrograde AAVrg and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...integration of cargo without using screening markers. Capability to construct a strain library with different ... that same year. Her research is focused on the escape mechanism of genome editing in microorganisms which...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...areas associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout...behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface. The ...

  8. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...study diseases such as cancer since mice better recapitulate the complex interactions between cancer cells...studied in other model organisms that better recapitulate in vivo conditions similar to humans. For example...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe via biotin...probe that is cut will continue to flow and be captured by an antibody further along on the strip. Highlights...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...major equipment can be defined as equipment with capital costs greater than $5,000 but this can depend on...like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools as possible, this even includes...

  12. Weighing Reagents Protocol

    Type
    Protocol
    ...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

  13. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... cassettes, making them advantageous for small capacity vectors like AAV. In addition to the mammalian...Yang. “Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” ...

  18. Video Library

    Type
    Protocol
    ...Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos...standard laboratory procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...against common tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176...associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH alleles off or on enables...

  20. Using a Light Microscope Protocol

    Type
    Protocol
    ...digital cameras attached to them that allow you to capture images directly to a computer, but many microscopes... your drawing skills. Regardless of your image capture method, be sure to record the magnification used...
Showing: 181 - 200 of 401 results