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  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...telomeres and centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to...

  4. Working with Nuclear Receptors

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    Blog Post
    ...for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins... the genome to sense the impact of the total environment on the cell. The NURSA Consortium Now in its ...

  5. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    ...start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon ... If the customer service rep does not tell you upfront how long it will take to get an answer, consider...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... fidelity, while the 52-piece assembly of a lac operon dropped to 49% fidelity, with significant lot-to-lot...Assembly, a great place to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to...

  11. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or... Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. doi:10.3389/fpls.2015.01001 Ho...

  12. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  13. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...solution that has been thawed more than once. Fibronectin is another membrane-interacting protein that ... 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

  18. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024...science in the open The York Lab operates with a strong commitment to exploration and collaboration. Andrew...

  21. Pooled Library Amplifications

    Type
    Blog Post
    ... library in a lentiviral backbone might be more prone to recombination, while a library with a large backbone...transcription factor overexpression library) or is prone to recombination, we recommend amplification on ...

  22. Of Myc and Men

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    Blog Post
    ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron...

  23. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during... References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation...

  24. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  25. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

  26. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...

  27. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...

  30. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

  31. An Introduction to Adenovirus

    Type
    Blog Post
    ...exposure, a lack of immunity, and then finally a synchronized exposure under these conditions once the measures...proteins and downstream cellular events, all recruit strong innate and adaptive immune responses that inactivate...

  37. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...

  39. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...

  40. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...
Showing: 401 - 440 of 671 results