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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 499 results for: RAP

Showing: 221 - 240 of 499 results

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...it, marking it as GFP+. Quantification for such graphs could simply be the percentage of the total cells...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...

  4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and... GEmCherry2 reporter systems provide efficient, rapid, and real-time read-outs of Cas9/gRNA efficiency...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...students to explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students...Students then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...could greatly enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid...says Mellett. This would help the lab identify therapeutic targets in the future. Congratulations Mark Mellett...

  10. To Each HIS Own

    Type
    Blog Post
    ... isolated from a complex mix using affinity chromatography against an amino acid sequence, or tag, on ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... basic biological research as well as clinical therapy. These biological tools are useful because they...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...

  17. Engaging with science and society at pgEd

    Type
    Blog Post
    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...plans, or information briefs for policymakers. The rapidly changing landscape of genetics and our consistent...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...p24 levels in a viral prep is straightforward and rapid as there are several commercially available p24 ...predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957. PubMed Central...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... been removed. The degradation and reversal are rapid compared to other systems because the system modulates...Cleveland DW. Inducible, reversible system for the rapid and complete degradation of proteins in mammalian...
Showing: 221 - 240 of 499 results