...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...

...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...

...for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of .... Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

... much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

...other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

...implications for our public policies—many of which impact reproductive rights and justice. Whether it’s used...interests. Our marches across the world have had an impact already, gaining additional funding and the eyes...

... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983-4002...

...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding... to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad host-tropism....

...Dynamics in the Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. ACS Chem Biol. 2018 Jul 20...levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

... Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise DNA-targeting...

...Kits Kits are groups of plasmids that have been packaged together because they are intended for use as ...

...research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to the global benefit...

...protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors ...

...course-based undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study...

...Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael...

...specific vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

... survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2...