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We narrowed to 958 results for: Cre

Showing: 221 - 240 of 958 results
  1. Synthetic Biology - Overview

    Type
    Collection
    ...Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc...
  2. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...
  3. Promega Plasmid Collection

    Type
    Collection
    ...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...
  4. p53 Pathway

    Type
    Collection
    ...disrupt its interaction with negative regulators, increase its stability and DNA binding activity, and allow...
  5. Open Enzyme Collection

    Type
    Collection
    ...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI 165537 ...
  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Scientists have made many genetic modifications to create bacterial strains that can be more easily transformed... specific treatments have been discovered that increase the transformation efficiency and make bacteria...
  7. Fluorescent Proteins: FRET

    Type
    Collection
    ...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...
  8. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...it contains a high percentage of glycerol that increases the density of your DNA sample causing it settle...better resolution of bands? A few simple ways to increase the resolution (crispness) of your DNA bands include...
  9. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... as opposed to transiently-transfected cells, increases reproducibility, as it eliminates the variation... vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods...
  10. Pouring LB Agar Plates

    Type
    Protocol
    ...maximum of ~100 colonies Pro-Tip If you will be screening a large number of colonies, we recommend using...your antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll ...
  11. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...an antibody. This protocol will describe how to create a standard curve using known concentrations of ...be empirically determined. The dilution series created in section 1, step 2 is a suggested starting point...
  12. AAV Production in HEK293 Cells

    Type
    Protocol
    ...harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-fold ( Rego et al., ...sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-fold. See the recipe...
  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression... Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived Nanobodies ...
  14. Protocol - How to Design Primers

    Type
    Protocol
    ...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...
Showing: 221 - 240 of 958 results