We narrowed to 958 results for: Cre

...Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc...

...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies...

...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...

...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...

...disrupt its interaction with negative regulators, increase its stability and DNA binding activity, and allow...

...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI 165537 ...

...AAV44.9(E531D) The AAV44.9(E531D) serotype has increased transduction and lateral spread in the retina ...

...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...grow bacteria in liquid medium Watch the Video! Creating Bacterial Glycerol Stocks Store bacterial strains...

...Scientists have made many genetic modifications to create bacterial strains that can be more easily transformed... specific treatments have been discovered that increase the transformation efficiency and make bacteria...

...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...

...it contains a high percentage of glycerol that increases the density of your DNA sample causing it settle...better resolution of bands? A few simple ways to increase the resolution (crispness) of your DNA bands include...

... proteins in the recipient plasmid (if you are creating a fusion protein) Ideally, you will find two different... 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert...

... as opposed to transiently-transfected cells, increases reproducibility, as it eliminates the variation... vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods...

...maximum of ~100 colonies Pro-Tip If you will be screening a large number of colonies, we recommend using...your antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll ...

...an antibody. This protocol will describe how to create a standard curve using known concentrations of ...be empirically determined. The dilution series created in section 1, step 2 is a suggested starting point...

...harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-fold ( Rego et al., ...sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-fold. See the recipe...

..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression... Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived Nanobodies ...

...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...

... to do the spreading; the tip is gently bent to create an “L” shape, and then used like a cell spreader...

...better resolution of bands? A couple simple ways to increase the resolution (crispness) of your DNA bands include...