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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 482 results for: vent

Showing: 221 - 240 of 482 results

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...collaboration and education in protein science, IPI hosts events and training courses on technologies and laboratory... on the IPI website and stay informed on future events by signing up for the IPI newsletter. The collection...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...expressed for a short period of time, which would prevent the accumulation of unwanted mutations over time...upregulating HDR, NHEJ must be downregulated to prevent the introduction of new mutations by CRISPR; editing...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...interest, while miRNA target sites can be used to prevent expression in cell types not of interest. Systemic...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...develop transferable skills by attending career events or being involved in a grad or postdoc association...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...glutamate molecules are released in each synaptic event and they are rapidly taken up by postsynaptic receptors...iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...for genome sequencing or annotation (5). In the event that genome sequencing can be done, this technique...functional cell-binding proteins in the (all-too-common) event that homology-based searches return no candidates...

  10. Chemogenetics Guide

    Type
    Guide
    ...23303063 (Link opens in a new window) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez...Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...

  11. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled actin...Intensity and filament length traces for a different event where CP appears briefly then leaves. Images reused...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...time. One system you could use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized...and thus the highest frequency of genome editing events. Yamamoto Lab Multiplex CRISPR/Cas9 Assembly Kit...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...

  19. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...transfer buffers. To slow down smaller proteins and prevent them from over-transferring, increase the amount... away the SDS. To speed up larger proteins and prevent them from under-transferring, try increasing the...
Showing: 221 - 240 of 482 results