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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 121 - 130 of 677 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...33714753. Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing the Voltron2 voltage...performance.      Figure 4: Structure of Voltron or Voltron2 conjugated to a JaneliaFluor dye emitting...HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron, 111(10), 1547–1563... SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia... to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently..., are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure...

  2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Visualizing single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (...labeling of cortical neurons and their synapses.   How can you label a single neuron and its synapses...DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system works in ...neuroscience Manipulation of specific cell types in neuronal networks Double inverted vectors expressing light...manipulate specific cell types (populations) within neuronal networks in living animals. To accomplish such...electroporation. To achieve sparse labeling of individual neurons one could reduce the plasmids amounts to reduce...efficiency. However, if the cellular structure of a neuron and its synapse should be visualized at the same...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...linked to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage...specific neuronal subtypes was not available until now. In order to target specific neuronal subtypes ...type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein, the authors...interest. In order to improve the study of specific neuronal cell types involved in neurodegenerative diseases...), and astrocytic (gfap).This toolbox adds new neuronal tools to the expanding gateway compatible vectors...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ... Inhibition of neuronal activation is a little more complicated since basal neuronal activity in your...advent of optogenetics, ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test...actually stimulating or inhibiting the targeted neuronal population prior to behavioral testing. One method...a reliable and widely used molecular marker of neuronal activation and is a sufficient readout to proceed...readout may be necessary to prove inhibition of neuronal activation, but it may still be possible to use...example, as shown in this study [3], hippocampal neurons are activated when the mouse is placed in a novel... the authors were able to show that HR blocked neuronal activation in the novel context as determined ...

  5. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...expression in E. coli. This operon is under the transcriptional control of a strong constitutive promoter (...powerful tool used by neuroscientists to interrogate neuronal networks. A major drawback of this method is the... the stray excitation of neighboring neurons in opsin dense regions. To overcome this issue, Shemesh et... C-terminus of the protein. Unlike the diffuse neuronal expression observed with wild type CoChR, soCoChR...describe a novel system for the precise mapping of neuronal networks at the single-cell level. Various soCoChR...PMID: 29184208.        Synthetic decaffeination operon Article contributed by Angela Kaczmarczyk  Listen...Listen to the synthetic decaffeination operon podcast segment With the help of engineered microbes, used...

  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived... by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...similarity. For these three kinases, photoswitchable Dronpa domains were inserted at sites homologous to the...here. In addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et...proteins could potentially be created by inserting pdDronpa domains into loops of a protein of interest. If...

  7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the ...iteration of this technology in 2007. Used to label neurons in the cerebellum of mice, the team appropriately...2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent proteins are organized...the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as many as 16 copies of the injected ...entire length of the cell, even axons of up to 100 microns in length. Some challenges of the Brainbow system...cells, the expression level of these XFP is not strong enough for all applications. To resolve this, similar...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...’s lab has enabled progress on both fronts – as a genetic electron microscopy (EM) tag providing superior...presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity in ...Druckmann et al., Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal imaging Joshua...Howarth. Shining a light on channelrhodopsins – Chronos & Chrimson Through de novo sequencing of 127 algal...These tools include a pair of channelrhodopsins, Chronos and Chrimson, which have enabled the group to perform...perform independent two-color optical excitation of neurons. The paper describes five previously unknown ...and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue and green light-excitable and has faster...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://doi.org/10.1016/j.neuron.2025.05.002...different interneuron populations. The enhancers span all four major classes of GABAergic interneurons — those... TurboCas Enhancer-AAV viral preps targeting interneurons Glutamate indicators with increased sensitivity...(L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s...Here's what makes these new backbones special: Strong expression: A CMV promoter drives robust gene expression... a test drive! We validated viral packaging and strong expression after inserting EGFP and provide step-by-step...recombinant antibodies targeting Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling...

  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Additional considerations CMV General expression mRNA Strong mammalian expression promoter from the human cytomegalovirus...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor...promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter Constitutive  Contains...activate promoter. Ac5 General expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive...Drosophila. Polyhedrin General expression mRNA  Strong insect promoter from baculovirus Constitutive  ...calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression. Modulated by calcium and calmodulin...mammalian EF1a promoter. GDS General expression mRNA  Strong yeast expression promoter from glyceraldehyde 3...
Showing: 121 - 130 of 677 results