...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...

...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...

...mouse gene-targeting field. Mice have long been extremely useful in the lab – they are relatively small ...

...1740-1743. PubMed PMID: 17882145. 2. Zincarelli, Carmela, et al. "Analysis of AAV serotypes 1–9 mediated...

...anti-science activists. However, I must say that I was supremely proud of Nashville that morning. Despite the rain...

...quantitative Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences...

...kD single heavy domain fragments derived from a camelid antibody called a heavy chain antibody (HCAb). ...

...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...

... E. coli, the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae,...

...doi.org/10.1016/j.neuron.2021.03.011 Hanlon, K. S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves...

... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...

...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...

... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ...

...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should...

...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers...

... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...

... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T...

...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells...

... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect...

...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace...