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A Career in Grants: How To Become a Grant Professional

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Blog Post

..., mid- to late-career grant professionals have similar stories about a time when their passion for an ...
Plasmids 101: Protein tags

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Blog Post

...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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Blog Post

... experiences in research labs. What are some similarities and differences you have observed as you have...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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Blog Post

... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...
Negotiating Work and Life: How to Find the Joy

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Blog Post

...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an...
CRISPR 101: Any Base Transversion Editors

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Blog Post

...from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse instead of...
Twenty Years of Sharing: Expanding Accessibility

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Blog Post

...about them. As early as 2007, we began grouping similar tools together in what would eventually become ...
Twenty Years of Sharing: Addgene's Viral Vector Service

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Blog Post

...broadly useful vectors, a process that isn’t as simple as it seems. Take, for instance, the GCaMP-encoding...
Tips for Writing a Good Cover Letter

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Blog Post

...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...
Plasmids 101: Broad Host Range Plasmids

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Blog Post

...within the scientific community, as they allow for simplified transformation processes, and can be used by ...
Antibodies 101: Reading a Flow Plot

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Blog Post

...they did in their experiments and determine if a similar approach would be good for your work. Hopefully...
Plasmids 101: Gateway Cloning

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Blog Post

...into destination vectors. The cloning process is simple - no restriction, ligation or gel purification...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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Blog Post

... target genes using dCas9-based activators The simplest of the dCas9 activators consists of dCas9 fused...
To Codon Optimize or Not: That is the Question

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Blog Post

... Rostovtsev A, Santana-Quintero LV, Katneni U, Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

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Blog Post

...cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants...
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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Blog Post

... fascinating social behavior. For example, two similar species have very different social behavior. One...
Viral Vectors 101: Pseudotyping

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Blog Post

...intact SARS-CoV-2 virus. Scientists have been using similar pseudotyping methods with other viruses in a BSL...
Rett Syndrome: A History of Research and Therapeutic Outlooks

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Blog Post

...severity. This exciting finding injected hope that a similar reversal may be possible for children and adults...
CRISPR-mediated Plant Base Editors

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Blog Post

...enables us to target more than one genomic site simultaneously. These binary vectors could be used to ...
Mesothelioma - Causes, Symptoms, and Treatment

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Blog Post

... the symptoms of mesothelioma are usually very similar to the symptoms of other diseases, such as asthma...
5 Reasons to Use Reddit for Science Communication

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Blog Post

...avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out the r/Science...
Getting the Most from Your Lentiviral Transduction

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Blog Post

... for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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Blog Post

...Pubmed Central PMCID: PMC3552020. 10. Paladino, Simona, et al. "Protein oligomerization modulates raft...
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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Blog Post

... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...
Hot Plasmids: Summer 2024

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Blog Post

... damage or permanently alter a gene product; it simply turns the target protein expression off. Plus, ...
An Integrin Antibody Toolkit from IPI

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Blog Post

...integrin subtype, even when in competition with other similar integrins, as reported by IPI and the Springer ...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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Blog Post

...pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...
March for Science

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Blog Post

...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate...
CRISPR 101: Homology Directed Repair

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Blog Post

...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...
Harnessing Bacterial Toxins for Allelic Exchange

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Blog Post

...system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli...
Quick Guide to Near-Infrared Fluorescent Proteins

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Blog Post

...). Modern state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a single gene to...
Transferable Skills Guide: Time Management

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Blog Post

...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...
Multiple Plasmids at a Low Price: Kits from Addgene

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Blog Post

...The Gomis-Ruth lab designed the pCri system to simplify the protein purification process by making it ...
Technologies Enabled by NanoLuc® Luciferase

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Blog Post

...brightness and improved maturation time compared to similar orange fluorescent proteins. In their work, the...
Plasmids 101: Dimers and Multimers

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Blog Post

...control when performing a diagnostic digest is a simple but effective method for detecting plasmid multimers...
Is this the right place for me? 8 tactics for choosing a lab

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Blog Post

...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...
Plasmids 101: Restriction Cloning

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Blog Post

...grow overnight cultures for DNA purification. The simplest purification you can do is a miniprep, but if ...
Live and Let Dye: Self-Labeling Protein Tags

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Blog Post

...Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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Blog Post

...mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus from the edited cells...
When Fidelity Matters: A frank discussion about ligase fidelity

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Blog Post

...extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP sites...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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Blog Post

...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...
Transferable Skills Guide: Teamwork

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Blog Post

...of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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Blog Post

...system once recombinant clones are generated. A simple way to do this is to express lambda red genes from...
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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Blog Post

...search for the perfect way to light up your science. Simon Moe is an Associate Product Marketing Manager at...
A Needle in a Base-Stack: Cas9 Structural Biology

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Blog Post

... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...
How to Deposit Your Plasmids with Addgene

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Blog Post

...a peer-reviewed journal. To start this process, simply enter your preprint title or a general description...
Deep Dive: qPCR

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Blog Post

...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...
Adeno-associated virus (AAV) Guide

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Guide

...help. Another virus, such as adenovirus or herpes simplex virus, or a genotoxic agent such as UV radiation...
Optogenetics Guide

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Guide

...and light oxygen voltage (LOV) domains function similarly to cryptochromes with light-induced changes in...

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