We narrowed to 717 results for: CaS;

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-” heterodimeric...

...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you ...

...pShuttle-CMV (Addgene #16403) plasmid with Gateway cassette Zhang 50958 ShuttleNSE For production of viruses...

...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

... N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization...

...Link opens in a new window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright,...

... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination...

...and differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact...

... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...

... of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was...

...markers for common cellular structures. In other cases, your experiment might rely on visualizing particular...

...either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting their genes of interest to generate...CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically inactive Cas9 (dCas9) with...pDGE Dicot Genome Editing Kit contains Cas9, Cas9 nickase, and Cas9 activator expressing plasmids that can...high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized...reported on a novel Cas9-based activator developed by the Church lab, termed “dCas9-VPR”, which facilitates...creating a new dCas9-VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into ... is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...

... potential applications. Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system...fully functional Cas9 upon dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal...specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This...gene activation when fused to dCas9. In the dCas9-SunTag-VP64 system, dCas9 is fused to a scaffold containing...domains onto dCas9 to generate a dCas9-VP64-p65-Rta fusion protein (referred to as dCas9-VPR) had a synergistic... can write a blog post about it! Split Cas9 system The Cas9 protein is composed of an n-terminal DNA... the bi-lobed architecture of Cas9 to engineer a series of "split" cas9 molecules that cannot function...

... gRNA, Cas9 binds the target site, but does not cut either strand of DNA. Traditional Cas9 regulators...capability, and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains...importantly, most Cas9 orthologs are less well characterized than the conventionally used SpCas9 (derived from...to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative nuclease... a potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance...repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9 is still able to bind DNA ...on rare occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make...

... can occur due to prolonged Cas9 expression     The requirement for Cas9 transcription and translation...consisting of the Cas9 protein in complex with a targeting gRNA, to your cells of interest. Cas9 RNPs are capable...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required and...long-term expression of Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the ...express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s ... of Cas9-gRNA RNPs by specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant...for experiments where stable Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein...

...The popular CRISPR base editing technique uses dCas9/Cas9 nickase fused to a cytidine deaminase for targeted..., a base editor containing high fidelity Cas9 variant HF-Cas9. They subsequently tested the original BE3...Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3... pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3  VQR-Cas9 (NGA)  Lower off-target activity than...-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3  VRER-Cas9 (NGCG)   ...85174  pBK-YE1-BE3  SpCas9 (NGG)  Editing window ~2 nt  85175  pBK-EE-BE3  SpCas9 (NGG)  Editing window...85176  pBK-YE2-BE3  SpCas9 (NGG)  Editing window ~2 nt  85177  pBK-YEE-BE3  SpCas9 (NGG)  Lower on-target...

...cells stably expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure... Figure 1: Cas9 expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting... Cas9 delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In these...with too many Cas9 integrations per cell, and my stable cell pool had wildly variable Cas9 expression (... variable Cas9-expression was across the different cells (Figure 3).   Figure 2: Cas9 expression...to assay Cas9 activity in my stable A549 cell pools. Using this virus, it’s expected that Cas9 activity...gene. So, cells with no Cas9 should appear green and cells with functional Cas9 should appear less green...