...nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E, Möglich A (2013...

...3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This toolbox...

...Sensitivity in Western Blotting. Journal of Pharmacological and Toxicological Methods, 64, 233–237. https...

...cotransfected with these constructs, providing the machinery that brings the sgRNA/cargo RNA to the appropriate...

...the antipsychotic clozapine and seems to be pharmacologically inert in mice and rats. However, CNO is back-metabolized...neurons through manipulation of ion channels arePharmacologically Selective Actuator Modules (PSAMs, pronounced...respond to specific small molecules termed Pharmacologically Selective Effector Molecules (PSEMs). PSAM...pre-requisite for mimicking clozapine’s actions? Psychopharmacology. 178:451–460. PMID: 15765260 (Link opens ...cAMP Production in Striatopallidal Neurons. Neuropsychopharmacol. 38:854–862. PMID: 23303063 (Link opens ...arrestin-biased designer G protein-coupled receptor. Mol Pharmacol. 82:575–82. PMID: 22821234 (Link opens in a new...

...using a sequencing machine that recognizes the different fluorescent labels. The machine detects which fluorescently...replication (by bacteria) by recruiting DNA replication machinery. The ori is critical for the ability of the plasmid... in the bacteria by bacterial transcriptional machinery. Multiple Cloning Site (MCS) Short segment of ...promoter is designed to recruit transcriptional machinery from a particular organism or group of organisms... when sequencing is not 100% efficient and the machine cannot distinguish between the 4 labelled nucleotides...

...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...direction based on the emission(s) read by the machine. Controls for cell sorting methods Flow cytometry...overlap to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...the virus depends on the cell’s DNA replication machinery to synthesize the complementary strand, transgene...

...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...

...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...