We narrowed to 417 results for: NUC

...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...

...take the nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus is still ...

... base editors probably due to the presence of nucleosome or other proteins. You can detect base editing...PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using hybrid prokaryotic and vertebrate...

...profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed.... 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature...

...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...

...unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID...selection systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID...

...fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize ...mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...

...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...

... of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation ...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

...include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...

...hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The...

...eukaryotic process where approximately 20-30 nucleotides of double-stranded RNA (dsRNA) results in downregulation...functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence of 2-FA,...

...antibody to detect various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently...staining should have high affinity and specificity to nucleic acids, with minimal background staining. Additionally...

... PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas...application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October ...

... drive expression, is it necessary to add a G nucleotide to the start of my target sequence? A3: The human...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. https...

...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...

...project aims to generate and characterize single nucleotide variant lines in an isogenic cellular background...

...repeatedly the reactive groups on a nucleotide when adding a nucleotide one at a time. The main property ...more than one nucleotide at that position termed a mixed site. One can also use a nucleotide-based molecular... Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction.... They are synthesized chemically by joining nucleotides together. One must selectively block and unblock... the primer usually has stretches of several nucleotides. Also, both of the 3’ ends of the hybridized ...process. When designing, if unsure about what nucleotide to put at a certain position within the primer...molecular insert (inosine) instead of a regular nucleotide for broader pairing capabilities. Taking into ...

... at specific locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (...

...editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing...