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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 423 results for: NUC

Showing: 241 - 260 of 423 results

  2. Zinc Finger Consortium Reagents

    Type
    Collection
    ... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

  3. TALEN Expression Vectors

    Type
    Collection
    ...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ... PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas...application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October ...

  6. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... drive expression, is it necessary to add a G nucleotide to the start of my target sequence? A3: The human...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. https...

  7. Retrovirus Plasmids

    Type
    Collection
    ...are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-...

  8. Arf GTPase Family

    Type
    Collection
    ...centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of GTPases is regulated... regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ... plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New and Noteworthy: Use tau constructs...Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural...

  12. Rett Syndrome

    Type
    Collection
    ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    .... Dopamine-glutamate neuron projections to the nucleus accumbens medial shell and behavioral switching...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link opens in a...

  14. Protocol - How to Design Primers

    Type
    Protocol
    ...repeatedly the reactive groups on a nucleotide when adding a nucleotide one at a time. The main property ...more than one nucleotide at that position termed a mixed site. One can also use a nucleotide-based molecular... Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction.... They are synthesized chemically by joining nucleotides together. One must selectively block and unblock... the primer usually has stretches of several nucleotides. Also, both of the 3’ ends of the hybridized ...process. When designing, if unsure about what nucleotide to put at a certain position within the primer...molecular insert (inosine) instead of a regular nucleotide for broader pairing capabilities. Taking into ...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...enzyme cloning, TA cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden ... Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding...

  16. Bacterial Expression Systems

    Type
    Collection
    ... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...

  18. Ligation Independent Cloning

    Type
    Protocol
    ...will include the free nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase...without ligation. Because of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs... which a single dNTP is included to stop the exonuclease function of the enzyme and shift its activity...polymerase protocol), causing the enzyme to perform exonuclease activity until the first "G" in the sequence.... the polymerase reaction is favored over the exonuclease reaction, the polymerase will add back the guanosine...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they... they will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into...

  20. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...also requires two unique single stranded DNA oligonucleotide (oligo) primers, which anneal to the regions...and the hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is ...increases the error rate of the DNA polymerase. Deoxynucleoside Triphosphates (dNTPs): Are the building blocks...strands of the template DNA. They are strands of nucleic acid that are starting points for DNA elongation...
Showing: 241 - 260 of 423 results