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We narrowed to 257 results for: PORA

Showing: 241 - 257 of 257 results

  6. Deep Dive: qPCR

    Type
    Blog Post
    ...configurations can help you identify pipetting or evaporation errors. And if time allows, you can fit in a ...

  9. Optogenetics Guide

    Type
    Guide
    ...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...photoswitchable” proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...transcription of the full-length virus after it is incorporated into a host genome. This is often found in second-generation...cells, they kill off any cells that have not incorporated the lentiviral genome and the cells that survive...interest and contains the sequences that will be incorporated into the host cell genome but cannot produce...after one round of reverse transcription. After incorporation into a host cell, this deletion prevents further...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...cells, they kill off any cells that have not incorporated the retroviral genome and the cells that survive...interest and contains the sequences that will be incorporated into the host cell genome but cannot produce...after one round of reverse transcription. After incorporation into a host cell, this deletion prevents further...

  12. Molecular Biology Reference

    Type
    Guide
    ...vitro DNA replication but relies on the random incorporation of modified, fluorescently tagged bases onto...difference in this process occurs when the polymerase incorporates a fluorescently tagged nucleotide. Because these...halted once the fluorescently tagged base is incorporated. Sanger sequencing requires a lot of DNA because...

  13. Plan Your Experiment

    Type
    Guide
    ... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

  14. CRISPR Guide

    Type
    Guide
    ...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...

  16. Adenovirus Guide

    Type
    Guide
    ...first-generation rAdV vectors than later generations, which incorporate additional genomic deletions. Figure 2: First-generation...

  17. Antibody Guide

    Type
    Guide
    ...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...
Showing: 241 - 257 of 257 results