We narrowed to 269 results for: gnal

... the primary antibody and amplify the detection signal.   Analyzing the western blot Once visible, it’...

...lead to high background that obscures the desired signal. We have successfully corrected this by incubating...

...information regarding which regulatory proteins, signaling molecules, mutations, and environmental conditions...

... it gets exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair...

...expressed has threshold effects, such as with signaling molecules or transcription factors, or when the...

...research into its many roles in protein function and signaling networks continues to expand. The Rinehart and...

...and publications by members of the Division of Signaling and Functional Genomics at the German Cancer Research...

... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...

... volume. The consequence is that you can detect signal in thick specimens. See the video below for an ...

...absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast ratios...

...in energy transfer and thus, your specific FRET signal is unaffected  Due to these fundamental differences...

...of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then expression...

...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848...

...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...

... The Liu lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max...

... promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy... promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light...

...160437 pUCIDT-attL1-Worm ABeta-attR5 APP Cleavage signal Alzheimer's Nicholas Tolwinski 160541 pGS_101 APOE...

...antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific antibody...abundant target, it obscures fainter signals. Fluorophores can emit signals that overlap with each other. Choose... Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Fc region. This allows for the amplification of signals by having multiple secondary antibodies binding...sdAbs is usually achieved by conjugation of a signaling molecule directly to the sdAb, or by using a secondary... incorporated in the sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins...single arm of the Y). They can be conjugated to signaling molecules and are often used in clinical applications...

...classified by the signaling protein that the receptors couple to: Gq-DREADDs Gq-DREADDs signal through the ...Gi-DREADDs Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...Gs-DREADDs Gs-DREADDs signal through the Gαs G-protein and activate neuronal signaling by increasing intracellular...small molecules that do not affect endogenous signaling, therefore allowing precise control over the cell...then activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide...of biological processes, including initiating signaling pathways in inflammation and neurotransmission...couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD, termed...

...NpHR with addition of trafficking signal from Kir2.1 and ER export signal provide improved membrane targeting...sensors (genetically-encoded reporters of molecular signals). These areas have each since grown and developed... the absorption spectrum or adding trafficking signals to localize opsins to the cell membrane. Microbial...566 eArch 3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton...566 eArchT 3.0 ArchT with addition of trafficking signal from Kir2.1 566 Leptosphaeria rhodopsins Mac Light-activated...maculans 540 eMac 3.0 Mac with addition of trafficking signal from Kir2.1 540 Plan Your Optogenetics Experiment...FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of Avena sativa phototropin...