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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: genome

Showing: 261 - 280 of 535 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ... amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually...increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the ...

  2. CRISPR Guide Update

    Type
    Blog Post
    ...additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... a nuclease-deficient Cas9 can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein...protein, a fusion with GFP allows for detection of genomic locations (Chen et al., 2013). GFP fluorescence.... 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by an optimized CRISPR...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...concentrations in cells than existing sensors. Using genome mining and structure-guided engineering of potassium...engineering. Figure 5: Structure-guided design and genome mining both led to new sensors with lower binding...Potassium Indicators through Structure-Guided and Genome Mining Strategies. ACS Sens. 2022 May 27;7(5):1336...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ..., green chemistry and understanding the minimal genome. Many of these techniques require more complex ...Assembly® is a registered trademark of Synthetic Genomics, Inc. One or more of these products are covered...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade...This could also enable more convenient multiplex genome engineering of crops or allow for sophisticated...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... cell lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty ...progenitors into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques ...

  11. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...technologies to functionally investigate the human genome. Doench says he enjoys working on technology development...functional characterization of specific edits to the genome. Such characterization can be done in a low throughput...

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections...user forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting...

  14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...ion channels, despite having an otherwise normal genomic background, can even lead to the formation of whole...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...or if you want to amplify sequences from a large genome organisms like mouse or human. There are several...amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online...Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources...

  17. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...researchers have adapted the components for use in genome manipulation. Cre recombinase, originally named... sequence does not occur naturally in any known genome other than P1 phage, and is long enough that there...

  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...William RA, et al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed...Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from... Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...embryo derived-ES cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 ...predicted off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos...
Showing: 261 - 280 of 535 results