Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 279 results for: mel

Clear keywords
Showing: 261 - 279 of 279 results

  1. Protocol - How to Design Primers

    Type
    Protocol
    ...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should...

  2. Ligation Independent Cloning

    Type
    Protocol
    ...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...

  4. General Transfection

    Type
    Protocol
    ... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T...

  5. Immunocytochemistry

    Type
    Protocol
    ...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells...

  6. Lentivirus Production

    Type
    Protocol
    ... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace...

  14. Western Blot

    Type
    Protocol
    ...approaches. Last Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer...

  17. AAV Production in HEK293 Cells

    Type
    Protocol
    ...handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in ...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...DNA pool. Last Update: August 17, 2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells...
Showing: 261 - 279 of 279 results