We narrowed to 279 results for: mel

1. Protocol - How to Design Primers

   Type

   Protocol

   ...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should...

2. Ligation Independent Cloning

   Type

   Protocol

   ...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers...

3. Fluorescence Titering Assay

   Type

   Protocol

   ... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ...

4. General Transfection

   Type

   Protocol

   ... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T...

5. Immunocytochemistry

   Type

   Protocol

   ...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells...

6. Lentivirus Production

   Type

   Protocol

   ... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect...

7. Colony Formation Titering Assay

   Type

   Protocol

   ...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace...

8. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...regulations. Last Update: February 18, 2022 Workflow Timeline Day 1: Seed cells Day 2: Transfect cells Day 3...

9. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...column gradient for AAV purification. Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration...

10. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...be adapted to alternative cell lines. Workflow Timeline Day 0: Seed and transduce Cells Day 2–3 (am): ...

11. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...should select an annealing temperature based on the melting temperature (Tm) of the portion of the primer that...

12. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ...in science. Last Update: June 14, 2023 Workflow Timeline Day 1: Run SDS-PAGE and stain gel Day 1 or later...

13. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...affinity purify recombinant antibodies. Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer ...

14. Western Blot

    Type

    Protocol

    ...approaches. Last Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer...

15. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: ...

16. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ...individual cells with cloning cylinders. Workflow Timeline This protocol begins with a stable cell pool. ...

17. AAV Production in HEK293 Cells

    Type

    Protocol

    ...handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in ...

18. CRISPR Library Amplification

    Type

    Protocol

    ...DNA pool. Last Update: August 17, 2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth...

19. Lentivirus ddPCR Titration

    Type

    Protocol

    ...regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells...