We narrowed to 275 results for: mel
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TypeProtocol...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells...
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Lentivirus Production
TypeProtocol... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect... -
Colony Formation Titering Assay
TypeProtocol...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace... -
Transfection for Recombinant Antibodies
TypeProtocol...regulations. Last Update: February 18, 2022 Workflow Timeline Day 1: Seed cells Day 2: Transfect cells Day 3... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...column gradient for AAV purification. Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...be adapted to alternative cell lines. Workflow Timeline Day 0: Seed and transduce Cells Day 2–3 (am): ... -
Plasmid Cloning by PCR (with Protocols)
TypeProtocol...should select an annealing temperature based on the melting temperature (Tm) of the portion of the primer that... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...in science. Last Update: June 14, 2023 Workflow Timeline Day 1: Run SDS-PAGE and stain gel Day 1 or later... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...affinity purify recombinant antibodies. Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer ... -
Western Blot
TypeProtocol...approaches. Last Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: ... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...individual cells with cloning cylinders. Workflow Timeline This protocol begins with a stable cell pool. ... -
AAV Production in HEK293 Cells
TypeProtocol...handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in ... -
CRISPR Library Amplification
TypeProtocol...DNA pool. Last Update: August 17, 2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth... -
Lentivirus ddPCR Titration
TypeProtocol...regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells...