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We narrowed to 677 results for: RON

Showing: 141 - 150 of 677 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...cell types (astrocytes, microglia, neurons, etc.) and even neuronal phenotype in a mixed population (Burger...have experienced that some vectors are much more prone to recombination and can exhibit recombination levels...your cells.  Some promoters, such as CAG are very strong and may require less viral particles or relatively...tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes...vectors for off-target expression.  Some classes of neurons transiently express TH during development or have...there is promoter activity. Additionally, some neurons express low levels of GFAP, which can lead to off-target...off-target expression of the packaged transgene in neurons and glia.   Check the recombination rate The first...

  2. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...their environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact...receive the product? How can we consider the environmental impact of those processes? Who even regulates...years since the creation of the United States Environmental Protection Agency, which started making regulations... can choose products that are better for the environment without sacrificing performance or quality. To...destined for a landfill. However, Triumvirate Environmental developed Red2Green, a program that uses this...lumber for things like truck bumpers, plastic adirondack chairs, and synthetic turf. I was truly amazed... a practice which we now realize is a major environmental disaster. We should continue to examine our ...

  3. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this...be used to drive Cre expression specifically in neurons. AAV Delivered Cre - A useful tool for neuroscientists...way. For example, say you would like to label neurons with mCherry and you already have a transgenic ...entirely new mouse line with mCherry controlled by a neuron specific promoter or you could breed your Cre-dependent...line with a separate line that expresses Cre in neurons, but both of these options would be very time consuming...and therefore mCherry will only be expressed in neurons. Indeed, this AAV provides you with a means to ...expression of any Cre-dependent gene of interest in neurons. This ability to be paired with any Cre-controlled...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Manon Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group at UCSF. It’s been only a few ...-- all while maintaining social distance.  From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy...scientists from more than 20 labs to form the QBI Coronavirus Research Group (QCRG) to do just this.  In under... York. We want YOU to join the fight against coronavirus We are just at the beginning of understanding...stick together (6 feet apart), to fight the new Coronavirus. To help get you started, here are a few key ...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since the publication...

  5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ... make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to oligomerize when...To do so, she generated fusion proteins in which Dronpa domains flanked either side of an enzyme’s active...ultraviolet light exposure caused oligomerization of the Dronpa domains thereby occluding the enzyme active site...the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back on....optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase its.... She additionally showed that these enhanced “pdDronpa” domains could be placed in amino acid loops on...iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin has been...

  6. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...proteins, work in different neurons and neuroglia, and have different effects on neuron activity (firing or inhibition...in a specific neuronal subtype in the brain that can either activate or inhibit neurons. You can find ...DREADDs for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki...chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics uses genetically... small molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal...concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.     References...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO) CRISPR Library. This...for neuronal systems. To develop a new single-component tool for magnetogenetic control of neurons, the...sequences outside of their endogenous chromatin environment and relies on the fact that enhancers function...J Genet Genomics. 2016. PubMed PMID: 26887506 Toronto KnockOut (TKO) CRISPR library In order to identify...Natl Acad Sci USA. 2016. PubMed PMID: 26884157 Aronshtam, et al. Nucleic Acids Res. 1996. PubMed PMID: ...but turns more red in lower (e.g. vacuolar) pH environments that inactivate SEP and leave only DsRed to ...the Co-IP compared of a control Co-IP indicates stronger interaction. The system has been demonstrated ...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...inducible degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron (AID) system...Tracking cell-cell interactions New auxin inducible degron system for C. elegans New CRISPR plasmids New items...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...   Schematic showing the auxin-inducible degron system. Image from Ashley et al., 2021.   The...spatiotemporal expression pattern and an auxin inducible degron (AID) sequence attached to your protein of interest...presence of auxin, this TIR1 complex binds to the degron sequence ultimately leading to the degradation ...the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins. Degradation of your ...

  9. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...activity of neurons on or off using pulses of light. By activating a neuron or set of neurons, you can see...activate a set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness...independently control the activity of two populations of neurons at once. Addgene spoke to Boyden about this latest...contributes to behavior or pathology, and by disabling neurons you can figure out what they are necessary for....examples where you want to activate two sets of neurons and see how different parts of the brain or the...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Much later, it was shown that putting the operon under a strong promoter results in high R-body expression...machines that can extend and retract over many microns in response to pH changes without the need for ... are composed of 4 proteins encoded in a single operon and were first expressed in E. coli in the 1980s... scaffold that changes shape in response to environmental conditions. More speculatively, R bodies might...of armor to protect their host from predation (Petroni et al 2000). With a better understanding of how...new ways to interact mechanically with their environment.   Jessica Polka is a Postdoctoral Research ... Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & ...
Showing: 141 - 150 of 677 results