We narrowed to 293 results for: CRISPR-Cas9

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Bacteria CRISPR Plasmids: Bacteria...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Edit Purify RNA Targeting Resources The following CRISPR plasmids have been designed for use in bacteria...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: ...

...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for ...

...Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing...Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

... other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools...

...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest...

...cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs ...

...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science , ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...

...at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO cell...

... comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit...

...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519...deposited their CRISPRoff system — a programmable epigenetic editor consisting of dCas9 fused to a DNA ...variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9, allowing them to be ...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...

...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type ...