We narrowed to 363 results for: gne

1. DNA Purification Without a Kit

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   Blog Post

   ...RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based method was...

2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   ...spectral range. Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell...

3. Lentiviral Vector Uses and Overview

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   Blog Post

   ... knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested...

4. CRISPR Antimicrobials

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   Blog Post

   ...currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system to resensitize antibiotic-resistant...

5. Cpf1: A New Tool for CRISPR Genome Editing

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   Blog Post

   ...to examine Cpf1 off-target editing. First, they designed gRNAs with single and double mismatches throughout...

6. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   Blog Post

   ...the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and can offer...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ...array of GFP-based fluorescent biosensors has been designed to detect a variety of intracellular conditions...

8. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ...those seen when using a plasmid backbone that was designed specifically for transient expression (find some...

9. Transfection for Recombinant Antibodies

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   Protocol

   ...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

10. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...promotes scientific sharing The EDGE Program is designed to expand the number of systems that can be studied...

11. Hot Plasmids - August 2020

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    Blog Post

    ... adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow of nanobody expression and...

12. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...early engagement with UPenn to our launch of the redesigned Data Hub in 2022. It’s influenced the way we ...

13. Hot Plasmids Spring 2024

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    Blog Post

    ...separation of the multiplexed elements. The team designed sgRNAs that include an internal barcode and separated...

14. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...all 101s on a particular topic into beautifully designed and easy-to-reference eBooks. Figure 3: Global...

15. Hot Plasmids: Fall 2024

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    Blog Post

    ...specifically for C. elegans (Cao et al., 2024). The team designed coding sequences for mScarlet3 and mScarlet-I3...

16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...Going Deeper: Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry...

17. Plasmids 101: Plasmid Incompatibility

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    Blog Post

    ... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...

18. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...code and have each developer work only on their assigned area. It’s natural that some of us have more in-depth...

19. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...included in the library. Safe-targeting guides were designed to target genomic sites with no annotated function...

20. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ...viewing a multiplex sample, the cut site will be aligned to the most 5’ cut site.     Indel Distribution...