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...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media...

...more suited for prediction of very diverse edit types and cell contexts. Figure 1: High-throughput... diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016/j.cell...

...light does not overlap in wavelength. In many microscopes the filters are not narrow enough to distinguish...Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent ...

...Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

...contained a viral envelope to dictate the tropism, or types of cells the vector could infect. Finally, the transfer...envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary cells or...

...on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their phages ...Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria. It’s a...

...location, location, location There are many different types of samples at Addgene - DNA sent from depositors...barcodes additionally allow us to store multiple types of tube for each plasmid we have at Addgene. Each...

...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles... mesothelioma make it different from many other types of cancer. Mesothelioma is considered an orphan ...

...regions. They also don’t give any clues about the types of neurons involved or whether their involvement..., et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute ...

...surprising because in vivo transduction of AAV serotypes generally tracks between strains of mice. This...between these two mice might drive their divergent phenotypes. Two labs, the Deverman lab at the Broad Institute...

...mesoderm is what gives rise to different tissue types in the developing embryo (Azplazu and Frasch, 1993...mutants vaguely resemble a bagpipe and historically, bagpipes were made using the stomachs of different animals...

...enhanced editing efficiencies in a number of cell types at different genomic loci — in some cases, over ... Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid protein, one of...

...collaborate whenever it faces an important challenge. The types of projects mentioned above are just examples, and...leverage the preferences of different personality types, and to devise strategies to be prepared for addressing...

...variety of photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...supports a variety of processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering...

... career scientists aren’t trained to have these types of negotiations. They usually turn into “win-lose...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all ...

...transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing...Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. ...

...for related studies in other brain regions, cell types, and tissues. Find cell-surface HRP plasmids here...vicinity of any one of five(!) different cancer types, the tumor size was greatly diminished, and tumor...

...unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical...

...protein regions available for antibody binding, or epitopes, differ between a protein in its native state ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...

...strong opsin expression in specific neuronal cell types then? This is where FLEx enters the scene. FLEx:...switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping." Journal...