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We narrowed to 517 results for: pes

Showing: 281 - 300 of 517 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...collect will depend on the serotype, as some rAAV serotypes are abundantly released into the culture media...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...control methods and compare the commonly available types of degron tags. What is a degron tag and why should...options! Below we will review the 4 most common types of degrons and some of the nuances to each one. ...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...light does not overlap in wavelength. In many microscopes the filters are not narrow enough to distinguish...Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent ...

  5. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...contained a viral envelope to dictate the tropism, or types of cells the vector could infect. Finally, the transfer...envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary cells or...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    ...on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their phages ...Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria. It’s a...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...regions. They also don’t give any clues about the types of neurons involved or whether their involvement..., et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute ...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...enhanced editing efficiencies in a number of cell types at different genomic loci — in some cases, over ... Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid protein, one of...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...immunotherapy than ever before. Although different types of cancer have different challenges and obstacles... mesothelioma make it different from many other types of cancer. Mesothelioma is considered an orphan ...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...collaborate whenever it faces an important challenge. The types of projects mentioned above are just examples, and...leverage the preferences of different personality types, and to devise strategies to be prepared for addressing...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...variety of photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...supports a variety of processes in different cell types. One great way to do this is to use CRY2-CIB1 clustering...

  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... career scientists aren’t trained to have these types of negotiations. They usually turn into “win-lose...sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all ...

  17. Antibody Guide

    Type
    Guide
    ...multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei...binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on constant...region. Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling... applications, and is covered in the following Isotypes section. The variable region of the antibody is...“heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing...Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. ...

  19. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...protein regions available for antibody binding, or epitopes, differ between a protein in its native state ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...
Showing: 281 - 300 of 517 results