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We narrowed to 677 results for: RON

Showing: 151 - 160 of 677 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...from a state of chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation ...cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from ...researchers are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...about alcohol and obesity as driving factors for chronic inflammation which in turn suppresses cancer immunosurveillance...surface receptor PD-1. This immunosuppressive environment in turn may represent a favorable state for tumor...Resources. Studying the immune system and tumor microenvironment Tumor Immunosurveillance and Defense With ...Friedmann-Morvinski about tools used to analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras...

  2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...our observations at the electron microscope showed us that, in monkey neurons, the large majority of the...are powerful tools to modulate the activity of neurons and other brain cells. Since the opsins or chemogenetic... we used AAV viral vectors to express hM4Di in neurons of rhesus monkeys or mice (Galvan et al., 2019)...found a larger proportion on the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small...hM4Di‐mCherry at the plasma membrane in monkey neurons, we next hypothesized that a different tag fused... of interest the membrane.   Figure 1: Electron microscope images showing ultrastructural localization...protein of interest, an alternative could be bicistronic expression of the fluorescent protein with the...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons. Your choice of AAV will depend upon... thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at ...post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University... These AAVs may move toward the cell bodies of neurons (retrograde transport) or towards the synapse (...a larger amount of vector using a serotype with strong CNS tropism (4). If axonal transport is not desirable...neuroanatomical tracing (11). With the ability to produce neuron specific transfection using Cre-lox, this new method... method could allow for labeling of neurons showing a very specific phenotype. Previous neural tracing...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...plasmids: Luc (intronless; CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I...Luc-I intron is a chimeric β-globin/immunoglobulin intron that has been commonly used in constitutive splicing...Multimerization via the SunTag system also shows strong upregulation of gene activation when fused to dCas9... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...induced-pluripotent stem cells (iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in...in mammalian cells, yeast, and Drosophila. Synchronization of secretory protein traffic in mammalian cells...disrupts this interaction and thus triggers a synchronous release of the reporters which can then be tracked...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...variety of applications, including detection of environmental pollutants, drug discovery, and disease diagnosis...instead of protein-based fluorophores, including: Environments with higher temperatures, since proteins can...Jonathan. “RNA Fluorescence with Light-Up Aptamers.” Front Chem. 2016 Jun 28;4:29. PubMed PMID: 27446908. PubMed...systems using immobilized aptamers.” Biosens Bioelectron. 2011 May 15;26(9):3725-36. PubMed PMID: 21419619.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...assay for prion diseases diagnostic”. Procedia Environmental Sciences. Volume 12, Part B, 2012, Pages 1348...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... pSC101 copy number is regulated by iterons. RepA binds to iterons and recruits host proteins to the ori...is initiated at the ori and may or may not be synchronized with the replication of the host cell's chromosomal...RepA binds to directly repeated sequences called iterons located near the ori and directly interacts with...check, high copy numbers can allow RepA to link iterons from two different plasmids, forming a pair of ...either of them (Park et al., 2001). The number of iterons encoded on a given plasmid influences how high ...host, as no additional components are necessary. Iterons are not the only tool to stringently regulate plasmid... numbers, RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing...

  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...factor RepA binds to the iteron to initiation replication. However, in iteron-containing plasmids,  RepA... regulation system involving antisense RNAs or iterons that inhibits replication when copy number in the...   Figure 1: The replication problem. Iterons are repeated sequences within the origin of replication...del Solar et al., 1998). There are two models for iteron-based plasmid replication control that are not ...the second model, termed the “handcuff” model, iterons of two separate plasmids bind to the same RepA ...these plasmid types? Read our Plasmids 101: Environmental Plasmids blog). Scientists have designed small...replication and transfer systems and host taxonomy. Frontiers in Microbiology 6: . https://doi.org/10.3389/fmicb...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...tobacco. Studying gene function in C. roseus is strongly limited by the availability of methods. Infection...reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful...days in the dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium...Agrobacterium, but not the plant. For example, we have seen strong expression of the GUS gene with the commonly used...Agrobacterium. This can be easily avoided by the use of introns, which will be removed from the transcripts in ...plants, check out this interview with the Nicola Patron, who shared the MoClo plant kit via Addgene. For...well working protocol in your lab, it can become a strong tool for your research. Feel free to add further...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...neural fibers developed by the Anikeeva lab are microelectronic fibers made from thermoplastic polymers that...desired thickness. The fibers are drawn with microelectronics included in them for downstream control – ...trended with expression of the opsin in the target neurons, validating the functionality of these inputs and...Success! They also observed reliable optically evoked neuron activity for at least several months after implantation..., P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable Neuromodulation... Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical Society Reviews...A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable wireless modulation of gut and...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated... exposed to blue light, this channel activates neuronal activity by opening and allowing positively charged...channels (rhodopsins) either excite or inhibit neuronal activity upon exposure to light within a specific...hyperpolarize the cell (Mattis et al., 2012). Bicistronic vectors combine excitation and inhibition by ... when you want to activate or inhibit specific neurons without needing to consider secondary signaling... off-target activity. Whether you are studying neuronal signaling or cancer pathways there are a number...2022). Red Light Optogenetics in Neuroscience. Frontiers in Cellular Neuroscience, 15, 778900. https://...
Showing: 151 - 160 of 677 results