...mission is to Accelerate research and discovery by improving access to useful research materials and information...Can Use the Viral Service in Your Research Using Virus in Your Research - A Primer for Beginners Resources...lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided valuable insight into AAV...focus on assisting scientists and accelerating research and discovery, while also pushing us to challenge...Visit Our Viral Vector Pages Find Virus for Your Research ...

...selecting which particular AAVs to use for your research. Below I’ll provide some tips that will help you...think about how to get started with AAVs. Since my research is rooted in the CNS, my focus will be mostly ...tolerated, then axonal transport is fine and a researcher should aim to inject a larger amount of vector...kB [9]).     4. The Future What new types of research may be enabled by the use of AAVs? 1) Combined...potential problem in many experiments, a group of researchers has identified this feature of AAVs as a new ... to study neural control. Surprisingly, little research has compared and contrasted optogenetic and chemogenetic... the most appropriate vehicle for this type of research, as they are safe to use in vivo. 3) The use ...

...Gary McDowell, executive director of Future of Research. On December 1st 2016, many postdocs working more...reversing these decisions entirely. At Future of Research, we had been collecting data and documenting the...blogpost and more recently in a paper published in F1000Research on November 2016. Due to the nature of the postdoc...was granted nationwide. Again, we at Future of Research believe that, while the mandate has been removed...what their institution is doing from Future of Research over social media, not from their institution)... currently the Executive Director of Future of Research. He is interested in advocating for and with junior... him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional Resources on the Addgene...

...materials, Sonali Roy, a postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB ...organized a panel to discuss reproducibility issues in research. But he realized that it wasn’t enough to discuss... that running interactive workshops to train researchers on tools and best practices could be an actionable... practices being adopted by a wider group of researchers. Generating material to empower others to practice... the materials in one place and better serve researchers at all levels of the scientific community. Lessons...interactive workshops that train and provide researchers with tools and best practices could be an actionable...

...by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM... different gRNAs. It has been suggested that researchers should use ~400X more cells than gRNAs in a screen...are addressing a well-defined, hypothesis driven research question. Examples of custom CRISPR screens Differentially...analyses like RNAseq are getting lower and lower, the research community is generating a massive amount of descriptive...such data. These targeted screens can allow a researcher to test which of the differentially expressed...embedded. These were kindly donated by six different research constellations: Doench/Root (2), Zhang (3), Wu...screen! Final note I am very grateful to the researchers letting us use their reference libraries thereby...

...longer than we’ve been studying it and, now, researchers are exploring ways to bolster and modulate our...protein blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73...to activate them are a few methodologies being researched. Apoptotic and necroptotic tumor cells have also...engineering have played a huge role. For instance, researchers are genetically altering tumor cells and immune... treatments enter the clinic, physicians and researchers are learning much about how treatment regimens... made more effective. Some important factors researchers are beginning to focus on include: Cell heterogeneity... important questions in this exciting field of research. One of the most important: How will cancers evolve...

...than cell cultures do, and tired researchers are accident-prone researchers. Note that safety policies will...have become a regular part of basic biological research as well as clinical therapy. These biological ...in non-target cells, including yourself as the researcher.   Figure 1: Separation of retroviral ... delivered, that is a higher risk cargo for a researcher to come into contact with than GFP. Similarly...need to be done in a biosafety cabinet. Many researchers choose to wear eye protection when they are working...likely to want to cut a few corners. For many researchers, viruses might be the most dangerous things they...

... involved in developing tools and solutions to research problems. This movement calls itself the Open ...and is part of a bigger movement in a variety of research sectors to provide open source technologies and...and spaces where interested parties can do research. This interest manifested itself in the creation of...collaboratively to solve, we want to focus our research on worm locomotion, but our worms are constantly...automated microscope  to keep our worms and our research goals in focus. Developing these skills relies...submit our new strains back to the CGC for other researchers to use. Although we haven’t yet made our final...University of Victoria. After a decade at Microsoft Research working on speech recognition, he returned to ...

...show you how Drosophila can be used to push your research in new and exciting directions. Drosophila are...the fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially...accessible resource out there to help keep your research moving. Below, you’ll find some great introductory... resources. As you dive deeper into Drosophila research, you’ll find more resources that may be more tailored...look up information about your fly gene using the search box in the upper right hand corner. They have video...Drosophila reagents are generously shared among the research community. They maintain many fly stocks that ...stocks are available at Bloomington, but you can search for various fly reagents on the Bloomington website...

...While much of CRISPR research has focused on genome editing, numerous discoveries have been made using...But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused on transcriptional activation...antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9 with the bound genomic locus...flavors to this methodology. Using this technique, researchers have begun to make important discoveries in various... region, other forms of enChIP, and additional research conducted in the Hodaka Fujii Laboratory, please...enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136. PubMed PMID...the analysis of bacterial genome functions." BMC research notes 11.1 (2018): 387. PubMed PMID: 29898790....

...immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers are also working...vector history and components In the early 1990s, researchers developed viral vector systems based on retroviruses...response, it should come as no surprise that researchers are interested in developing these vectors for...vectors have become standard in gene therapy. Researchers have also developed integrase-deficient lentiviral...come a long way since the 1990s, both in terms of research impact and safety. Many of Addgene’s most requested...Virus Biosafety Find Lentiviral Vectors for Your Research  ...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... is clearly a good idea to be diligent in your research, remember that you will not be a graduate student...training. Further, even for those students and researchers who do take the initiative to develop themselves...have a well-groomed LinkedIn page. Here you can search for jobs, post your CV, and, most importantly, ... can be critical to a focused and effective job search. As with any online forum, there is an etiquette... someone a lot older than you are; try using ResearchGate to see where other graduate students and post-docs...

...a little over half of our orders coming from researchers outside the USA, having some materials available...data and resources to increase accessibility to researchers around the globe. Addgene’s educational resources...become our collection pages. These pages allowed researchers to easily access and compare their options for...expression types, and more, all of which help a researcher understand if a material is right for their experiment...2023, we had over 900 posts, which were read by researchers all over the world — and we really do mean all...educational resources’ value is feedback from the researchers who use them. The best part of any conference...crowdsourcing experimental help. We love it when a researcher reaches out to ask if they can help us create...

...depending on the part of the company involved: Research & Development (R&D) teams at pharma companies ...companies carry out preclinical and clinical research to discover and test the best new therapeutics. Consultants...among physicians and scientists about clinical research, drugs, the diseases they can treat, and diagnostic...client might need to get a large amount of market research done to inform a go/no-go decision in a very short...PhD, an MD, or someone trained in any aspect of research in biology or medicine you will bring an essential...global health organizations, investment and equity research firms, biotech start-ups, venture capital firms...consultants. I have even seen consultants go back to research and bench science, with a newfound appreciation...

...Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic...the amount of money wasted in basic oncological research due to contamination are in the tens or hundreds...lines deposited with them and used by different researchers are free of contamination and match their original...perhaps a working cell bank has been made by a research institution (an excellent practice), or maybe ...Warwick University, gaining extensive cell culture research experience through working for a variety of pharmaceutical..., HeLa cells, and cell culture contamination." Archives of pathology & laboratory medicine 133.9 (2009...

... you’re working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds...have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries, pooled barcoding... virus background.           Maybe your viral research has taken you in the direction of COVID-19. We...find plasmid tools to accelerate your COVID-19 research.  Download the red COVID-19 background.       ...Here, you can browse plasmids by field, organism, research technique, and more. Download the green DNA background...whether you use molecular biology tools in your research or not! And learn more about the variety of careers...

...to share curated information about AAV usage in research applications. Scientists around the world can ...Reports receive a DOI for citation, crediting researchers when their data is used by the community to drive...in materials sharing, information sharing, and research reproducibility while expanding our suite of materials... materials and services to support more research and innovation around the world. As always, we seek to...opening up science to help create new knowledge and research opportunities. I have spent the last 9 months ...same is Addgene’s commitment to supporting the research community through sharing science and distributing...

...Morgan Model organisms are vital tools used by researchers around the globe. These organisms share many ...most preferred mammalian model organism of many researchers: the mouse (Mus musculus). Mice have many advantages... human diseases and have successfully helped researchers develop a variety of therapies. For example, ...fly. The fruit fly has been used in scientific research for over a century but was brought to the forefront...Morgan, who is known as the “father” of Drosophila research. Morgan discovered that genes were found within...is a commonly used model organism in scientific research. Yes, the same kind we use in breads and other...molecular biology reference Find plasmids for your research ...

...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...about IF, direct and indirect. For direct IF, researchers use a single antibody to visualize their target...their results and corresponding protocols. Other researchers may also have tested an antibody in IF and will...organism that is underrepresented in the biomedical research community. You may have to take some risks on ...provide you not only answers to your most pressing research questions, but also stunning images (am I the ...results as artwork at home?). Take your time to research antibodies, protocols, and appropriate controls...Additional Resources on the Addgene blog Learn about research applications of antibodies in Introduction to ...

...over three decades. Widely used across various research applications, they have become indispensable tools...and organisms. They can be used for a variety of research purposes, like creating stable cell lines, delivering...vectors work with regards to integration, and how researchers have modified them to prevent this integration... DNA… forever!  This can be an advantage for researchers looking to generate long-lasting changes in cells...genome (Figure 1). IDLVs were first discovered by researchers investigating HIV integrase mutants in the early... choice for certain types of clinical or basic research applications. Considerations for IDLV use If you...you know how IDLVs could be useful in your own research, we wish you the best of luck with any experiments...