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  1. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction...of that particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation...

  3. Antibodies 101: Validation

    Type
    Blog Post
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation...victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains a mixed population...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  7. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  9. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially...

  20. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...
Showing: 301 - 320 of 414 results