Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 587 results for: ret

Showing: 301 - 320 of 587 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...

  3. Five Popular Model Organisms

    Type
    Blog Post
    ... (Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found...21415126. PubMed Central PMCID: PMC3082451. Pfeiffer, Barret D., et al. "Refinement of tools for targeted gene...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ...

  7. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...%}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid option...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...composition.  So back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA...

  12. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing...

  13. Synthetic Biology - Overview

    Type
    Collection
    ...Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. ...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

  15. Antibody Production

    Type
    Collection
    ... ensure that potency and specificity have been retained. If the target antigen is not endogenously-expressed...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote...
Showing: 301 - 320 of 587 results