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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 533 results for: try

Showing: 301 - 320 of 533 results

  3. Synthetic Biology - Overview

    Type
    Collection
    ...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository of community-created...

  4. Microbiology Resources

    Type
    Collection
    ...Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis...

  5. Open Enzyme Collection

    Type
    Collection
    ... synthetic biology, DNA origami, immunology, biochemistry, and more. The foundational technologies enabled...

  7. CRISPR Guide

    Type
    Collection
    ...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (... & Liu, D. R. (2018). Base editing: precision chemistry on the genome and transcriptome of living cells...

  8. Antibody Guide

    Type
    Guide
    ...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...

  9. Brain Initiative Collection

    Type
    Collection
    ...dendrites under the control of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Opti-MEM, Thermo Fisher 31985-070 0.05% Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH 7.4...Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~2 min. Neutralize trypsin by adding 10 mL of DMEM Complete...new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from 2 x T-175...cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend cells from the CS2. Cells should...60 mL PBS. Aspirate PBS and add 35 mL of 0.05% Trypsin/EDTA. Wait ~2-3 minutes for cells to detach. Gently...until no live cells can be seen when stained with Trypan Blue. Pellet cell debris by centrifugation at 3900...

  11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue ... Isolate cells from the stable cell pool by trypsinization and break up any cell clumps into individual...expanded but before they become over-confluent, trypsinize the cells and expand them to larger culture dishes...the independent monoclonal lines. Pro-Tip When trypsinizing cells from the 96-well plate, use a microscope...detached from the plate and can be isolated. When trypsinized, detached cells will exhibit a rounded morphology...

  12. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...containing the trypan blue. Pipette 10 times to mix. Load 10 µL of the cell suspension/trypan blue mix into....45 µm PES filtering system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium...flask 5–10 times before sampling. Transfer 10 µL of trypan blue into a clean microcentrifuge tube. Vortex ...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...indicating the breakdown of ancestry associated dependencies across ancestry groups (right).    Seeking...divided into those of African ancestry(orange) and those of other ancestry groups (grey). Misek and... representation of every ancestry group, they should have found more ancestry-associated dependencies ...any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated...Institute, where an exploration into the effects of ancestry and germline sequence variation in cancer cell...down unexpected paths. Though this research uses ancestry groups rather than self-reported race, it highlights...screens in DepMap to see if they could identify the ancestry of the cell lines (European, East Asian, Indigenous...

  14. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... to travel meant the accessibility varied country to country.   Figure 1: Number of countries Addgene ...Views from a country are represented in blue; darker blue indicates more views from that country.   While ...Views from a country are represented in blue; darker blue indicates more views from that country. Stats and...the USA, but our materials are available to any country that we can export to — so far, we’ve shipped materials...distributors Distributor Partnership Start Date Country Served Beijing Zhongyuan 2012 China ...distributors broadly helped increase access in the country, and we can see a growth in material distribution...distributor helped solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive...

  15. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...available to you including careers in academia, industry, non-profit, and government sectors.  Within these...area, then the research role could be the point of entry into that field. In this post, we discuss the following...endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...start working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B ...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher, 12605010) Ethanol, VWR, ...each well. Detach cells by incubating with 200 µL TrypLE for 1–2 min. Resuspend cells in 500 µL DMEM complete...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry : Official...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In ...Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129...Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional ...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the surface...used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could be cells or ...investigate in each panel. An advantage of flow cytometry is the ability to use multiple fluorescent signals...compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies can be used...within the cell or tissue as well. Similar to flow cytometry, you’ll use antibodies to stain for markers of...can be analyzed using techniques such as flow cytometry. Conclusion Whew! Now you know that antibodies...

  19. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing...shared her flow cytometry expertise in two posts. Flow Compensation and Flow Cytometry Controls Priyamvada... guide to understanding your flow data. Flow Cytometry Readouts Toj, a summer intern who shared their...validation expertise. Antibody Validation for Flow Cytometry Rob Hurt, whose expertise and suggestions were...

  20. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...schools to promote chemistry degrees and provide advice to students who want to study chemistry. The society... scientists are introduced to the biology and chemistry societies that will form the core of their scientific...with the goal of facilitating networking between chemistry students, especially those studying to earn a ...events throughout the year, including a Christmas Chemistry symposium and a career night. The society also...students in society and to support iGEM alumni-industry networking to promote the creation of start ups...
Showing: 301 - 320 of 533 results