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We narrowed to 677 results for: RON

Showing: 161 - 170 of 677 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is... to study how protein turnover is regulated in neurons. Protein turnover is the combined effect of protein... I wanted to measure it’s levels in its normal neuronal context. In other words, I needed a method for...for visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover... whole population of one particular protein in neurons or in any other cell type. However, we cannot distinguish... newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897-905. PubMed...Robert H. Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability...

  2. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...might think that being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a...about seven different skills to you, you are not wrong. Problem-solving overlaps heavily with other skills...core part of our academic training.  Developing strong problem solving skills is just a matter of applying...As such, scientists are uniquely prepared to be strong problem solvers both in and out of the lab. The...position. Of course, there are limits to how far strong problem solving skills alone can take you - just...effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset is a part of ... Ashley Waldron...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ... photons are absorbed by matter, they promote electrons within atoms to a higher energy state. This process... called excitation. After a short period, the electrons revert to a lower energy state, whereby a photon...those of absorbed photons. When photons excite electrons of a fluorophore, they can do so at a range of...by the cytometer. Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few...absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure 2: Excitation... real-life advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal...and FITC an organic molecule), their bleed is so strong that these spectra almost overlap. Hence, they ...

  4. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid is a ...through transformation (cellular uptake from the environment), transduction (viral infection by bacteriophages...their host organisms to thrive in competitive environments. The genes encoding these functions form the...less aggressive ways to survive in competitive environments — like by living where no one else wants to....others can’t. This ability to endure a harsh environment is distinct from the benefit of resistance plasmids...lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444. https://doi.... environmental plasmids...

  5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...be used for sparse labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic...broad number of cell types, such as inhibitory neurons, rods, cones, or retinal ganglion. Rather than ...that produce sparse labeling would be useful for neuronal tracing experiments where lower levels of labeling...labeling makes it easier to trace a neuron’s projections. Generating animal models: Transgenic animals allow...for retinal gene therapy Find more about precise neuronal targeting using AAV technologies Browse all of... H.P., Krol, J., & Roska, B. (2019). Targeting neuronal and glial cell types with synthetic promoter AAVs...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... stem cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane...synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ...ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after...antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies against important...

  7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable...injected the spike protein from two different coronaviruses into a llama over several days and isolated ...sybodies that hypothetically contain ones that bind strongly to your target protein. The Seeger lab designed...enrichment step for identifying sybodies that bind strongly and specifically to the protein of interest. The...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ... carriers collection Find other COVID-19 and Coronavirus Plasmids & Resources ...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...only a few, very strong associations.   Unexpected results Their results showed 96 strongly correlated genetic...accessible to the research community. Sean Misek and Aaron Fultineer in the Beroukhim and Boehm labs pulled...either European or East Asian ancestry, Sean and Aaron knew that their study had significant statistical...mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad’s Pattern team,...varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella...Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, Adam Brown,...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...copy. This is a versatile technique, and is less prone to off-target mutations. Ultimately, successful ...frequently requires considerable optimization to ensure strong counter-selection. Anecdotally, there is also a... evolved specifically for toxicity would allow stronger counter-selection than one merely co-opted toward...rhamnose regulon, we inserted a copy of rhaS with a strong synthetic ribosome binding site (using the online...isothermal assembly master mix. Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM...Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990. PubMed PMID:...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed PMID...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...extremely strong affinity for one another. Actually the bond between them is one of the strongest noncovalent...that scientists began to exploit biotin and its strong interaction with avidin for molecular biology and...are useful for biotinylation in different microenvironments. There are also reversible and cleavable biotinylation...attached to a photoreactive arylazide group. Under strong visible light, the arylazide group becomes reactive...Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular Biology of Biotin Attachment ... PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic biotinylation of proteins...
Showing: 161 - 170 of 677 results