We narrowed to 376 results for: fus

...axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport...

...minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF...

...Uncx Mouse Mouse IgG2a 114547 Anti-Mitofusin-2 [N171/17.4R] Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin...SNAPAP Human Mouse IgG2a 140072 Anti-Mitofusin-1 [N111/24R] Mitofusin-1 Mouse Mouse IgG2a 140073 Anti-Pannexin...Mouse IgG2a 225377 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a...IgG2a 225378 Mitofusin-1 (Mus musculus) recombinant monoclonal antibody. Mitofusin-1 Mouse Mouse IgG2a.../Tmem90b Rat Mouse 211603 Mitofusin-1 scFv [N111/24] N111/24 scFv Mitofusin-1 Rat Mouse 211604 Botch scFv...Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising a κ light chain...heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed by their insertion...

...element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain...reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation...-controlled transactivator (tTA) was created by fusing TetR with the activation domain of the herpes simplex...tetracycline-controlled transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline...3xFLAG_Twin_Strep Tet-inducible expression gene of interest fused with 3xFLAG and 2xStrep-tag from TRE3G promoter ...

...stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the...

...coli The Kondo lab expressed a cytidine deaminase fusion with Cas9 with a uracil DNA glycosylase inhibitor...

...dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation...mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes...

...TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited...6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction... 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts prefix to preserve...

...genetic material. Proteins are delivered to cells by fusing them with peptides that help mediate their transduction...

...Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Thomas Jessell...constitutive Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter... mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter...

...Tyler Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA...pLV-mCherry 3rd CMV driven expression of mCherry fusion. See 36083 and 36086 for other FP variants. Pantelis...Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein. Ethan Abel 135991 pSLCAR-CD19-28z 3rd ...

...library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the...expressing and purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite...

...150 µm to image a blot. This is because proteins diffuse semiradially during the transfer, making membranes...

... RNA (sgRNA), which is derived from a synthetic fusion of the CRISPR RNA (crRNA) and the trans-activating...components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the...This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return...

...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs).... Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates...

...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....with different fluorescent tags for you to create fusion proteins with your gene of interest. Most of these...

...bound by the gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification. Various...antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing...

...Resources Using catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which recruit...

... pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre...pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo...

...Tetracycline inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV...loss of dsRed and the activation of the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase...