Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 677 results for: RON

Showing: 171 - 180 of 677 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...neuroscience by granting targeted control over neuronal activity. While they serve similar purposes, both...scale of milliseconds, and can precisely link a neuron's activation to behaviors. Scientists continue to... mutated to have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer...Administering the drug once can result in several hours of neuronal excitation or inhibition. This sustained activation...tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel at discrete cell ...precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...monitoring the activity and biochemistry of living neurons in an effort to understand the connection between...available beam splitters. In addition to imaging neurons, Griesbeck adds that this color change has a particular...refined by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors allowed ...researchers to see tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes...transition metal ions, such as zinc, copper, and iron. “These are essential metal ions, but they are also...calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...sterilization as mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination! Tissue culture...shortly. Once they are present in the laboratory environment, they are difficult to eradicate. You can suppress...maintenance of the cell lines. Providing such an environment for the lab facility will not only reduce mycoplasmas... you are careful enough to provide a sterile environment for work, it will soon become a habit before ...separate footwear for the lab to avoid bringing in environmental contaminants.  Figure 2: An Addgenie...lines for assay or analytical purposes. Use 0.1-micron filters as are they are a better way of avoiding...avoiding mycoplasma, rather than using 0.2-micron filters, for media filtration. There is no 100% assurance...

  4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...reversibly degrade proteins of interest fused to a degron, a domain that induces degradation. In plants, ...ligase complex, promoting its interaction with the degron sequence and ultimately its degradation. While ... needed for AID. By tagging proteins with a 7kD degron called mAID and expressing the essential plant ...channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab and collaborators... cysteine.  With the introduction of MerMAIDs, neurons can be turned off quickly and with high precision... a valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS ...

  5. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...there was strong expression of Cpf1 and GFP in the targeted brain region and ~75% of neurons were co-transduced...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo. For these experiments, cells were ...-transduced with Cpf1 and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal service slams dumpsters...useful transferable skill. Being able to stand up in front of an audience and convey your ideas clearly and...project. So whenever you freeze, or when things go wrong, ask yourself, “What next step will help me achieve...term “diving board” so you end up acting it out in front of a very confused group of visiting Chinese undergrads...incorrectly on slides 8-16. Your notes disappear. The wrong version of your slide deck gets uploaded. Your mic...

  7. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...Tet-on transgene vector. Degron tags Tagging relevant proteins with a degron tag can provide inducible...removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)...SMASh degron tagging vector! Destabilizing domains Destabilizing domains (DD) are akin to degron tags,...

  8. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... RNA-sequencing to identify neuronal class and deep sequencing of neurons from each class to fully characterize...Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map & Network (CeNGEN) The CeNGEN...comprehensive gene expression atlas of every C.elegans  neuron. Scientists are currently doing this using two ...this system a modified TIR-1 protein can degrade degron-tagged proteins in an auxin dependent manner. This...Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed PMID: 30092212. PubMed...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials ...perceived signals to physical actions. Understanding neuronal pathways and the function of certain cells depends...be directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...membrane as well. Another flavor of these tools is Voltron, which uses rhodopsin as the voltage sensing component...voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It follows that...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...norepinephrine (NE) expressing neurons and GFP (green) to label neurons transduced by each serotype. ...challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type...express ChR2(H134R) in A2 norepinephrine expressing neurons. We tested four serotypes containing an expression...84%) were both excellent at infecting our target neurons (Figure 1). However, this one set of experiments...Perspectives on Data Sharing in Deep Brain Stimulation. Frontiers in Human Neuroscience, 14(December), 1–12. https...
Showing: 171 - 180 of 677 results