We narrowed to 494 results for: control
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TypeBlog Post...second major area deals in post-experiment quality control, a good example is CRISPR-GA. These tools assess...place to focus further development toward. Quality control is, however, not a focus of this blog post. I separate...
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Antibody Guide
TypeGuide...negative control. The negative control may be a no-antibody control in which a sample is processed without ...standard curve can serve as the positive control); and a negative control, a sample known not to contain your...interest. The negative control should be biological (no protein expressed), which controls for your antibody...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and a negative...addition to the control used in standard IP, ChIP should additionally use a beads-only control which runs ...the machine. Controls for cell sorting methods Flow cytometry requires a negative control, a small subset...basic steps needed for each application, important controls, and any special considerations to think about... -
Optogenetics Guide
TypeGuide...research utilize light to detect, measure, and control molecular signals and cells in order to understand... genetically-encoded tools for light-activated control of proteins; e.g., microbial opsins and optical...subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and off the light...illuminated. There are several different ways to control opsin expression. Generally, the most robust and...-ChR2 mouse, where ChR2 is expressed under the control of the vesicular gamma aminobutyric acid (GABA)...GABAergic neurons being activated by the light is controlled via the placement of the optic fiber. Using viral...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...fluorescent tools are used simultaneously to monitor or control different cell types or signals in the brain. For... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...be sure to include proper positive and negative controls as you get your assays up and running. Ed.'s ... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post... the details, but the developers have it under control. 9:30 AM--Back-to-back meetings. Meet with my boss... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post..., you’ll want the proper positive and negative controls for your PCR. Find the details and learn more... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...sequence is also found in the reporter gene). A control RFP expression plasmid. With this set up, all... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...protein. The expression of this fusion is under control of the arabinose-inducible promoter. Cells are ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...respectively, are used as positive and negative controls for proper ER structure. Based on these data, ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin promoter. Purpose of this... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...They might be researching enhancer elements that control the transcription of a gene a megabase away, or... -
Visualizing Protein Turnover In Situ
TypeBlog Post...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while having... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments. Find the scrambled... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...they worry about needing more study subjects and control groups. The important point, however, is that cost... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens ...