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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 494 results for: control

Showing: 341 - 360 of 494 results

  2. Antibody Guide

    Type
    Guide
    ...negative control. The negative control may be a no-antibody control in which a sample is processed without ...standard curve can serve as the positive control); and a negative control, a sample known not to contain your...interest. The negative control should be biological (no protein expressed), which controls for your antibody...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and a negative...addition to the control used in standard IP, ChIP should additionally use a beads-only control which runs ...the machine. Controls for cell sorting methods Flow cytometry requires a negative control, a small subset...basic steps needed for each application, important controls, and any special considerations to think about...

  3. Optogenetics Guide

    Type
    Guide
    ...research utilize light to detect, measure, and control molecular signals and cells in order to understand... genetically-encoded tools for light-activated control of proteins; e.g., microbial opsins and optical...subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and off the light...illuminated. There are several different ways to control opsin expression. Generally, the most robust and...-ChR2 mouse, where ChR2 is expressed under the control of the vesicular gamma aminobutyric acid (GABA)...GABAergic neurons being activated by the light is controlled via the placement of the optic fiber. Using viral...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer...

  4. Plan Your Experiment

    Type
    Collection
    ...CRISPR experiments and can be used as positive controls when working out a new protocol. Read more about...Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation. Cell , 159 (...

  5. Promoters

    Type
    Guide
    ...Transcription is tightly controlled in both bacteria and eukaryotes. Promoters are controlled by various DNA regulatory...vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase...a functional protein. Thus the promoter region controls when and where in the organism your gene of interest... are proteins which recruit RNA polymerase and control and regulate the transcription of DNA into mRNA...consists of the sequence TTGACA and this element controls the rate of transcription. Bacterial cells contain...Operons are a cluster of different genes that are controlled by a single promoter and operator. Operons are...operon in bacteria is an example of a negatively controlled operon. A positive repressible operon works in...
Showing: 341 - 360 of 494 results