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We narrowed to 677 results for: RON

Showing: 181 - 190 of 677 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D...techniques like super-resolution microscopy or electron microscopy. Since its introduction in 2015 by ...2010). How do you overcome this resolution limit? Electron Microscopy (EM) is one option, but the equipment...helped researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux... Ashley Waldron...

  2. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...biological studies, including investigations into environmental pollutants and health, embryo growth, brain ...example, specific tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers... for neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming...high-resolution mapping of the brain by uniquely labeling neurons. Zebrafish are also amenable to manipulation of...consequences of activating or silencing specific neurons in living larvae. In addition, zebrafish have been...PubMed PMID: 18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...make them ideal for studying in a laboratory environment. We’ve covered five model organisms in a previous...candidate for introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia... disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical...genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant...Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159/000323761...the Axolotl Model for Regeneration and Aging. Gerontology, 66(3), 212–222. https://doi.org/10.1159/000504294...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters..., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central...to target the central nervous system, including neurons, AAV5 is generally the best choice for targeting... is derived from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up ...

  5. Antibodies 101: Validation

    Type
    Blog Post
    ...molecule at all, leading to inconclusive results, erroneous conclusions, and wasted time. In fact, many have...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...that antibody works as expected, you will see a strong antibody signal in the retinal sample, but little... your antibody (B). Your results show a lovely, strong band from the cells transfected with your protein...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL... Ashley Waldron...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    ...mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse neuron without...optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts to engineer...of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with ...antibody anti-c-Myc [9E10] now available! by: Ashley Waldron The majority of Addgene's more than 50 antibodies...

  7. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems...smaller plasmids are formed. Viral vectors are prone to recombination In lentiviral vectors, recombination... elements. Knowing how to amplify recombination-prone plasmids and testing your prep can save you the ...H (2012) Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3...Genetic recombination of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular...

  8. What the HEK?

    Type
    Blog Post
    ... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great...researchers using the cell line. What the HEK is wrong with it?   All cultured human cell lines are susceptible..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389...

  9. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...equipment always helps. But how does one build a strong collaborative research team motivated by similar...your lab team and foster an effective working environment? Tom Ellis was offered a start-up package that...ways to improve the lab. “An effective working environment for research is totally dependent on communication...while others are more productive in a handsoff environment. “Treating people fairly does not mean equally...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

  10. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....
Showing: 181 - 190 of 677 results