We narrowed to 500 results for: RAP

1. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of editing frequency...

2. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding...

3. Communicating Your Science With Help From ComSciCon

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   Blog Post

   ...science communication comes with practice. You can bootstrap your science communication skills with training...

4. Why and how to start studying chromatin (with Addgene's help)

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   ...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI...

5. AAV Q&A with Tim Miles

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   ...discussed in our recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel ...

6. Hot Plasmids - February 2022

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   Blog Post

   ...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

7. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...small, avoidable mistake. Or worse, when you're trapped in a pattern of small errors that significantly...

8. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

9. Antibodies 101: Monoclonal Antibodies

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   ...hybridomas  Learn about monoclonal antibodies for immunotherapy Resource on Addgene.org Check out Addgene’...

10. New and Upcoming Viral Vectors - May 2020

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    Blog Post

    ...and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent ...

11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the spread of ...

12. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...tires give a better grip in the cold, but wear out rapidly in warm weather. Psychrophilic enzymes face very...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    .... You fill a column with OptoNB coated beads and wrap the column with blue LED lights. When you switch...

14. A Quick Guide to a Career in Software Product Management

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    Blog Post

    ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

15. Plasmids 101: Inducible Promoters

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    Blog Post

    ...inducer, like Tet systems, may be activated very rapidly. In contrast, promoter systems that require transcription...

16. Hot Plasmids - May 2022

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    ...likely to afford the ability to deliver more therapeutics to the CNS, and with reduced systemic side effects...

17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...three strategies described in this post in the graphic below - including some simple comparisons. The...

18. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...

19. Plasmids 101: Yeast Vectors

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    Blog Post

    ...prokaryotes. This includes but is not limited to: rapid growth, ease of replica plating and mutant isolation...

20. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ... desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have...