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We narrowed to 399 results for: PREP

Showing: 361 - 380 of 399 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...efficient generation of large-scale, high-titer preps. This is thanks to established retroviral production...

  2. AAVED

    Type
    Collection
    ...Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute... reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's publication...commitment to open science! Please see the meeting preparation section for how to best help contribute to this...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...PGRN GRP gastrin-releasing peptide BN, GRP-10, preproGRP, proGRP GUCA2A guanylate cyclase activator 2A ... OSMRB OSTN osteocrin MUSCLIN OXT oxytocin, prepropeptide MGC126890, MGC126892, OT, OT-NPI OXTR oxytocin...pro-melanin-concentrating hormone MCH PNOC prepronociceptin PPNOC POMC proopiomelanocortin ACTH, CLIP,...

  5. Cre-lox system

    Type
    Collection
    ... of choice. We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's...service provides high-quality and high-titer viral preparations of plasmids that are currently in our repository...

  6. Antibody Guide

    Type
    Guide
    ...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...

  8. Viral Vectors

    Type
    Collection
    ...array of research purposes. Ready-to-use viral preparations for select plasmids are now available through...

  9. COVID-19 Resources

    Type
    Collection
    ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...novel population of long-range inhibitory neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids. ID...

  12. Bacterial Expression Systems

    Type
    Collection
    ...ratio) Escherichia coli Christopher Voigt 65008 pCRISPReporter-mCherry Promoter activity Fluorescence (mCherry...

  14. CRISPR Library Amplification

    Type
    Protocol
    ...Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare, sterilize, and pour all LB Agar + Antibiotic...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically... high efficiency (1x10 10 cfu/µg) commercially prepared electrocompetent cells is strongly recommended...Antibiotic 65 mm Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000...access to sterile scraper and cold LB. Pro-Tip Prepare one to two more conical tubes on ice in case you...spread out the harvested cells further than four Maxipreps worth. Count colonies on the most dilute Petri.... It will be close enough for the purpose of a Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi...

  15. Western Blot

    Type
    Protocol
    ...determination. Prepare a 50:1 Reagent A to Reagent B dilution of the BCA assay. Prepare serial dilutions...Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer, block, incubate... Determine the total protein concentration and prepare the lysate for SDS-PAGE Determine the protein concentration...need to load a greater amount of total protein. Prepare sample for loading as follows: Add 10% v/v β-mercaptoethanol...samples for 10 min at 100 °C . Section 3: SDS-PAGE Prepare the precast gel as follows: Remove the gel from...instructions for your specific tank. Close the clamp. Prepare 1X running buffer as follows: Dilute 25 mL of 20X...for 15 min in 20% ethanol in deionized water. To prepare 20% ethanol, dilute 2 mL of ethanol into 8 mL of...

  16. AAV ddPCR Titration

    Type
    Protocol
    ...FAM-CACTCCCTCTCTGCGCGCTCG-BBQ- Reagent Preparation 1X PCR Buffer containing 0.05% Pluronic F-68: Prepare immediately before...cool. Prepare the ITR master mix in a microcentrifuge tube as shown below. For 8 samples prepare enough...4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials ready...sufficient pipette tips and reagent reservoirs. Pro-Tip Prepare the viral dilutions in a separate biological safety...(dilution BSC) than that used for master mix preparation and droplet generation (droplet generation BSC...well plate sealer by gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate...well freezer block and place in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ... virus on ice. Prepare the following dilution series of virus in DMEM complete: Prepare 7 microcentrifuge...Assay: RPP30, Human, Bio-Rad, 10031244 Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate...untransduced control. Pro-Tip For even seeding, prepare a batch for 10 wells with 3,000,000 cells in 13.5...concentration of each sample on a spectrophotometer. Prepare 25 ng/µL stocks of each sample. Samples can be ...immediately or stored at -20 °C until ready to use. Preparing for ddPCR Thaw samples, primers/probe mixes, and... plate sealer by gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a ...freezer block and set aside in the BSC to cool. Prepare the RRE/RPP30 master mix in a microcentrifuge tube...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI add...use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with EcoRI and NcoI: 1 μg miniprep DNA...Recommended Materials Material Vendor and catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #...based on your experiment Detailed protocols for preparing polybrene, protamine sulfate, and puromycin are... BL2 safety practices should be followed when preparing and handling lentiviral particles. Personal protective... 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution by dissolving 40g of LB powder...store at 4°C. Hexadimethrine Bromide (Polybrene) Prepare a 1mg/mL solution of polybrene (Sigma-Aldrich catalog...

  19. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...BCD TFX) to 37 °C. See below for other Reagent Preparation instructions. Wipe down all pipettes and equipment...equipment with 10% bleach prior to use. Reagent Preparation 100 mM Valproic Acid Dissolve 2.88 g valproic...Sterilize by passage through a sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C... Filter the solution through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX... reagents Store at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 ... Glutagro Store at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ...Use this protocol to prepare LB agar plates with antibiotic in your lab....samples are sterilizing in the autoclave, you should prepare your plate pouring station: Find an empty section...antibiotics, and a section for active pouring. Prepare your antibiotics. Prior to adding your antibiotic...1000x stock solution. For example: If you’ll be preparing plates with a final concentration of 100 ug/mL..., dissolve by vortexing, and filter sterilize. Prepare a water bath at 60 ℃ with sufficient water to submerge...Only the Resistant Strain Grows If everything was prepared properly, you should see growth of only the resistant...
Showing: 361 - 380 of 399 results