We narrowed to 368 results for: Pol

...exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity creates ...and thymine (T). TOPO cloning utilizes the Taq polymerase, which naturally leaves a single A overhang on...Additionally, the efficiency can vary depending on the polymerase used, and the single A overhangs degrade over...common molecular biology enzymes: 5' exonuclease, polymerase, and ligase. In Gibson assembly, DNA fragments...each other due to their homology. Second, DNA polymerase then closes the gap created by the 5’ exonuclease...reaction. In the presence of a single free dNTP, T4 polymerase will continue to function as an exonuclease until...complementary to the free nucleotide. T4 will resume its polymerase activity, add back the free base, and become ...

...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...

...resulting in excitation (depolarization) of the neuron. Inhibitory (hyperpolarizing) ChR variants Anion-conducting...optogenetics tool. They typically allow the fast depolarization of neurons upon exposure to light through direct...chloride channels, these variants result in the hyperpolarization of neurons. Examples of anion channel variants...NpHR (from Natronomonas pharaoni ), causes hyperpolarization (inhibition) of the cell when triggered with... a light-activated outward proton pump that hyperpolarizes (inhibits) the cell when triggered by green-yellow... robust optogenetic inhibitors that act by hyperpolarizing cells via high K + efflux. Synthetic light-...

... DNA polymerase then binds to the single strand of DNA. Using this as a template, the polymerase migrates... requires the four nucleotides (dNTPs), a DNA polymerase enzyme, the template DNA to be copied, and a ... DNA and acts as a starting point for the DNA polymerase. Thus to replicate a piece of DNA in vitro, you...

...increased nuclear export. Poly(A) signal in cis The polyadenylation or poly(A) signal aids in the nuclear...vector capsid. Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle...

...with up to eight transcription units. Yarrowia lipolytica Golden Gate tool kit Yeast Expression Tristan...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina...multi-gene constructs for integration into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast...

...vector capsid. Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle...

...j.cell.2014.09.029 PMID: 25307932 Hilton, I. B., D’Ippolito, A. M., Vockley, C. M., Thakore, P. I., Crawford...