We narrowed to 405 results for: expression vector

...a site within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be...sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...

...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Zebrafish"...Zebrafish". Cut ID Plasmid Gene/Insert Vector Type Promoter PI Publication Return to top CRISPR Resources Addgene...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Plant". Activate...CRISPR expression plasmids that have been designed for use in plants. CRISPR...

...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

...recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Parasite"....CRISPR expression plasmids that have been designed for use in parasites. CRISPR...

...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...

...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms...

.... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...

...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...

...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Xenopus". ...

... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "C. elegans...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Drosophila...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Yeast". Activate...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

...Example of viral targeting of cells expressing Cre AND Flp, here expressed through a double transgenic animal...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...and VCre to enable viral expression of molecular payloads in target populations defined by multiple parameters...understanding of neural circuit functionality. Precisely expressing these tools in well-defined cellular sub-populations... a single gene or projection). Intersectional expression approaches combine multiplexed recombinases, ... including Cre, Flp, and VCre to enable viral expression of molecular payloads in target populations defined...