We narrowed to 413 results for: his

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...

2. Engaging with science and society at pgEd

   Type

   Blog Post

   ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical...

3. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   .... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...

4. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   ...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

5. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently...

6. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

7. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred...

8. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

9. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   ... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...

10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ... vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate. These tags...

11. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    ...Plasmids for Mammalian Systems Learn about CRISPR History ...

12. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...

14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...

15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    ...they should not be relied on exclusively because historical studies of pathogen research have conclusively...

16. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ...

17. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    ...will bind to the column due to their N-terminal His14-bdNEDD8 tag. After several washes, this tag can ...

18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...

19. Getting to Know Expansion Microscopy

    Type

    Blog Post

    ... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

20. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...