We narrowed to 416 results for: iCre

...Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed...

..., injection of diffusible labels, or electron microscopy of stained sections, were limited due to low ...

...G., Tereshchenko, J., & Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity...

...conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block some amines needed for conjugation...In those cases, thimerosal can be used as an antimicrobial agent. Other chemical interferences with conjugating...strong interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in..., and wash. Preserve sample and image using a microscope. Special considerations Many of the steps in ...in this protocol vary depending on assay and microscopy techniques used, so be sure to read materials specific... flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647...

...differences in tropism between cell types (astrocytes, microglia, neurons, etc.) and even neuronal phenotype in...

...require help from a biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed...

... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...

...Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University...

...fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo....

...Engineering Resources on Addgene.org Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains ...

...particular ligand (such as when a component of a microbe or a cytokine is added to a cell). With a custom...

...tools derived from direct comparative analysis of microbial opsins. Nature methods. 2012;9(2):159-72. doi:...

... can be submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial...

...SNP aware”, “secondary structure aware”, and “microhomology aware”. Trends: Fewer tools are being released...

...Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular activities using light...

...facilitate cellular uptake of these dyes in vivo, microinjection, pressure injection or iontophoresis (applying...

...-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions ...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...classification of CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N...diverse bacteria with Mobile-CRISPRi. Nature Microbiology . 4 (2), 244–250. PMID: 30617347 Qi, L. S., ...

...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...available at Addgene. Optogenetics In optogenetics, microbial opsins and other optical tools developed by researchers...

...bacterial and fungal species for engineering non-model microbes. AspFlex Kit Bacterial Expression, CRISPR Veronica...

...Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression ...