Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 677 results for: RON

Showing: 11 - 20 of 677 results

  1. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...backstage team. Meet the microenvironment, a tumor’s backstage team. Microenvironments are cellular regions...Tumors are surrounded by microenvironments and what encircles a disease is highly prone to affect it. While...type in the tumor microenvironment is the immune cells. The contradiction is strong in this one. After...cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments have...Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009): 9-17...associated fibroblasts (CAFs) in tumor microenvironment." Frontiers in bioscience: a journal and virtual...precisely that for breast cancer. What are tumor microenvironments made of?  A tumor’s genesis is divided into...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...solution for discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic... et al., 2016, 2018).   How to use Tetbow for neuronal labeling To use Tetbow, you can introduce the ... is interested in developing genetic tools for neuronal circuit tracing.     Marcus Leiwe is an assistant...Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent...and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical...Read about Brainbow Learn about using AAVs for neuronal tracing Visit the viral vectors blog topic page...

  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting...folding at low temperatures, since mesophilic chaperones would not function well under suboptimal conditions...engineering a plasmid which contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica...the figure below shows, E. coli expressing the chaperones protein resulted in higher levels of growth compared...help foster a more productive and collaborative environment for research.  We’ve also involved ourselves ...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...and developed the SciNote Electronic Lab Notebook. Implementing an electronic lab notebook starts with ...should have functionalities such as: electronic signatures, electronic witnessing and audit trails. How ...write things down when doing scientific research? Electronic lab notebooks and researcher's needs The familiar...traceability and reproducibility of research. Electronic lab notebooks (ELNs) are here to make lab work...explored the barriers to adoption within academic environments to better understand the issues labs are facing...traceable. However, switching from paper to an electronic lab notebook requires time and knowledge. In ...recent article, we translated our knowledge of electronic lab notebooks into 9 questions that will help...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... the brain, a connectome that would detail how neurons form circuits and where they synapse. View the ...Golgi staining, injection of diffusible labels, or electron microscopy of stained sections, were limited due...inversion of the construct. Sanes and Lichtman used pronuclear injection to insert these Brainbow constructs...vast array of colors needed to mark individual neurons, the system also suffered from a number of limitations...the tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis... disproportionately expressed by a majority of neurons, limiting the number of distinct colors that could...through at least six generations. In addition to the neuronal pThy1-Brainbow constructs, Addgene also has two...

  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like...that go on between plants and their pathogens. As Patron explains it, her work has always been focused on...Modular Cloning (MoClo) Kit. What does it include? Patron: Sainsbury has a mandate to share and educate and...Addgene: Will the MoClo tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses...tips for those looking to get started with MoClo? Patron: The parts will be familiar to most plant scientists...there other tools you are working on for plants? Patron: Our lab and others at the Sainsbury are working... of engaging about science in that way for you? Patron: I started my Twitter account recently inspired...

  8. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...neuroscience with many brain regions, neuronal networks, and specific neuronal populations to consider. To tease...Making use of AAV’s selectivity for neurons: targeting defined neuronal populations using viral strategies... in neurons that project to different regions of the brain. A more locally defined set of neurons can ...tease apart how different neurons impact a certain output, neuroscientists have begun to use more precise...genetic tools with the manipulation of specific neuronal subtypes and determine how these changes affect...combinations of AAV-based molecular tools for targeted neuronal manipulation. The goal of the workshop was to ...practical guide which was recently published in Frontiers in Neuroanatomy.  Here are the three main takeaways...

  9. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...Elementary and Middle Schools   Image courtesy of Ron Mader, via Flickr, under the Creative Commons license...education that is provided through a structured environment such as the classroom or a defined learning program...
Showing: 11 - 20 of 677 results