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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 405 results for: pho;

Showing: 11 - 20 of 405 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore can emit...the photon budget and pushing the frontiers of fluorescence microscopy often requires more photons. For...cells can decrease the number of fluorophores, compromising the photon budget. Likewise, the switch from...desperate to extract a few more photons from a sample. Chemical fluorophores can be substantially brighter...more photostable than fluorescent proteins, providing a straightforward way to improve the photon budget...rhodamine fluorophores, such as the Alexa Fluor and ATTO dyes, with improved brightness, photostability, and... of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores are ...

  2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...the go-to method is to use spectrophotometry. Microvolume spectrophotometers make it quick and easy to ... jumping in — in the world of microvolume spectrophotometers, the Thermo Scientific NanoDrop is one of...details might vary.  How it works — briefly Spectrophotometers measure how light travels through a sample...contaminants, some of which are easier to detect by spectrophotometry than others. The ones most likely to impact...distinguishing between the two can be difficult by spectrophotometry. RNA tends to yield a higher A260/280 and ...Concentration and Purity with Microvolume Spectrophotometers. NEB Tech Note (2019).  Loughrey S and Matlock...Nucleic Acid Samples Using the NanoDrop One Spectrophotometer. Thermo Fisher Scientific Tech Note (2016)...

  3. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...Listen to the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which...., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain from A. sativa into a loop...ratio, high sensitivity, responds quickly, is photostable, and is compatible with optogenetic control. ... Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed...temporally control cellular processes in vivo. Many photoactive proteins exist in nature, but developing them... either the light- and dark- states of the photoswitchable cPYP, which changes in conformation in response...bind to only the light- or dark- state of the photoswitchable proteins offers a powerful approach to control...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific) - PPP3CA protein phosphatase 3 (formerly...IP1, IP2, IPD2, NEMO INPP5D inositol polyphosphate-5-phosphatase, 145kDa MGC104855, MGC142140, MGC142142...FLJ26625, JTK8 MALT1 mucosa associated lymphoid tissue lymphoma translocation gene 1 DKFZp434L132, MLT... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...and cytoplasmic domain, (semaphorin) 6D FLJ11598, KIAA1479 SEMA7A semaphorin 7A, GPI membrane anchor (...BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic protein 10...MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein 2 BMP2A BMP3...

  5. Genetic Code Expansion

    Type
    Collection
    ... Expansion Accessible Using Phosphoserine to Study Protein Phosphorylation Rinehart Lab reagents tRNA/... mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic... pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang Ai 73544 pEvol-pAcFRS...91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P....91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P.... PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser...140022 pAS_4xMma PylT_FLAG-PcKRS PcKRS M. barkeri photocaged-lysine Mammalian TAG Simon Elsaesser 140023 pAS...

  6. Antibody Guide

    Type
    Collection
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Webb Phosphate FRET-based phosphate sensor (FLIPPi) A novel analytical method for in vivo phosphate tracking...Ca(2+) indicator with enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. doi: 10.1117...29;7. pii: 32638. Kai Johnsson Phosphate Scaffold for inorganic phosphate biosensor Development of a Reagentless.... Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based calcium integrator...calcium sensor with exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025... jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Nat Methods...localized zinc sensor ZIBG2 Genetically Encoded, Photostable Indicators to Image Dynamic Zn(2+) Secretion ...

  8. Ras Pathway

    Type
    Collection
    ... Protein phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent...receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein...PIK3R5 PIK3R6 Also known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...state, regulated by the addition and removal of a phosphate group. Under normal physiological conditions GTPases...thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating...DUSP2 DUSP3 DUSP4 DUSP5 DUSP6 Dual specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2...G1, G2, G3: non-catalytic subunit gamma PTEN Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related...

  9. Optogenetics AAV Preps

    Type
    Collection
    ... Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Deisseroth... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...

  10. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are...690/717 (after photoactivation) 3.2 pPAiRFP1-N1 PAiRFP2 692/719 (after photoactivation) 3.0 pPAiRFP2-N1... imaging using far red, near-infrared, and photoactivatable fluorescent proteins. Plasmid...imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent...The following far red , near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal...piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find...
Showing: 11 - 20 of 405 results