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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 545 results for: SEC

Showing: 21 - 40 of 545 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Voeltz 49155 mCh-Sec61 beta Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...S0896-6273(20)30398-6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent...Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional... pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV...755-60. Loren Looger , Eric Schreiter Calcium Intersectional viral expression of sRGECO (constitutive or...Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional... pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial and cytosolic...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...Rluc), and NanoLuc® Luciferase (Nluc). Luc2 is the second-generation version of Firefly luciferase that ...transcriptional reporters. However, they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, making time course experiments...size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available – these attributes...applications. Browse the Collection Highlights section to learn more. Highlights of Luciferase Tools in...ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of renilla luciferase Liqun Luo 51970...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...mCherry Monoclonals, Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...express a gene; however, we have included a function section for if you want to stably express an insert using...vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54,...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, ...

  6. Antibody Production

    Type
    Collection
    ... and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared...untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized, and labeled with the...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern is compared...antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared to an...

  7. AAVED

    Type
    Collection
    ...achieve targeted neuronal manipulation (aka intersectional control). The end result of the meeting will...protocols showing examples of AAV strategies for intersectional control. Please see our list of suggested materials...representing a reference for AAV use in neuronal intersectional control. Through your participation in this...open science! Please see the meeting preparation section for how to best help contribute to this publication...

  8. Arf GTPase Family

    Type
    Collection
    ..., Gateway GEF Iqsec1 9922 1114 Gateway GEF Iqsec2 23096 1488 Bacterial (pET) GEF Iqsec3 440073 1182 GEF...

  9. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

  10. All Antibodies

    Type
    Collection
    ...experimental details in the Antibody Applications section of our product pages. We also include negative ...affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more about antibodies and...

  11. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...

  12. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...future publications. For your Materials and Methods section: "The plasmid kit used in this publication was ...(Addgene kit # 1000000013)" For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... been designed for use in Drosophila and other insects.... been designed for use in Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce...

  15. Cre-lox system

    Type
    Collection
    ...recombinases that are less toxic in Drosophila hsp70 Insect Rubin 32606 Ttr:Cre Cre expressed in visceral endoderm...EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...dSTOP-loxP>-myr::GFP Cre dependent myr-GFP expression Insect Rubin 51503 AAV pCAG-FLEX-tdTomato-WPRE Cre dependent...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoprotein expression in rEcoli XpS in the Methods section link below (Methods for iSPI, rEcoli XPS, pSerOTS-C1...phosphosite DNA library can also be introduced into a second expression vector encoding a split mCherry fluorescent...

  17. CRISPR Guide

    Type
    Collection
    ...CRISPR in your own research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR...Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding. In this...have been developed, including editors based on a second ADAR variant that displays improved specificity...Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans...Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For multicistronic expression...

  19. Genetic Code Expansion

    Type
    Collection
    ...M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Bacterial TAG P. Chen 91706 pCMV-MbPylRS(DiZPK)...M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG...

  20. Brain Initiative Collection

    Type
    Collection
    ...channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin...channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus ...
Showing: 21 - 40 of 545 results