We narrowed to 844 results for: Tat

...mismatch/mutation may be the result of a mis-called peak in the trace file. If the mutation is not an...such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting one or more...Addgene sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence...

...and plasmid recombination can all impact the representation of individual plasmids in the pooled library...individual libraries may require modifications dictated by the originating laboratory for optimal results...adequate NGS based QC to ensure no change in representation compared to the pre-amplified stock. A last...note that NGS should be performed to ensure representation is maintained. Maxipreps - Less is more: Do...

...by a ‘CAC’ codon that is converted to a ‘TAC’ or ‘TAT’ after a C-to-T base editing event. This edit changes...Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR... is a bicistronic construct that consists of a mutated mCherry and a downstream constitutively active ...the eGFP reporters the Harris lab individually mutated three codons in eGFP to eliminate fluorescence....eGFP reporters rely on the correction of point mutations in eGFP that results in the restoration of fluorescence...GFP construct, that contains an ACG start codon mutation, and integrated it into human and mouse cells ...events in vivo. As GO relies on the correction of a mutated start codon to initiate protein expression, it ...

...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor ...missense mutations located in the MBD, and truncations prior to the NID are more severe than mutations further...to their laboratory website. Cell Line Mutation (DNA) Mutation (protein) Sex Source of Material Publication...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...meet diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2...erminal D omain (CTD) The most common missense mutations cluster in the MBD and NID demonstrating the importance...

...Barros Lactate/Pyruvate Lapronic FRET-based sensor for Lactate/Pyruvate Ratio Imaging of the Lactate/Pyruvate...(4):525-531.e4. Dmytro Yushchenko Lactate Laconic FRET-based lactate sensor Barros Lab Laconic Plasmids...Felipe Barros Lactate Expresses eLACCO1.1 fluorescent biosensor for extracellular L-lactate, in neurons,...extracellular L-lactate. Nat Commun. 2021 Dec 6;12(1):7058. Marie-Eve Paquet , Robert Campbell Lactate Lindoblum... fluorescent biosensor for lactate Green fluorescent protein-based lactate and pyruvate indicators suitable... cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded ...Oct 23;9(10):e110600. Isei Tanida Electrostatic potential Quantitative measurements of membrane charges...

...recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items...Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful...producing a functional molecular tool (C,F). Implementation The following resources may be of interest ...developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...

...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be...target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence (8–10 bases at the... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting...

..., deletion, or translocation) depends on the orientation of each target site (see Figure 1). Figure 1:...Recombination outcomes are determined by target site orientation. A) If the target sites (loxP) are on the same...(Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer... lox511, loxN, lox66/lox71 Dre rox TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer...site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8... F3, F5, FRT14, FRT15 minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and... example, iCre or FLPo in mammalian cells may facilitate higher rates of recombination. Gene Expression...

...contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present...Photoactivatable (Off to On) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission ...maximal value. Structure describes the oligomeric state of the protein. Many FPs are monomers at low concentration...vector type, popularity, and more. Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...Bacterial Expression Return to top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...Bacterial Expression Return to top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...Mammalian Expression Return to top Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

...71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus ...References for each serotype can be found below under Citations. AAV2(Y444F) The AAV2(Y444F) serotype is demonstrated...from the AAV2 capsid and contains the following mutations: Y444F, Y500F, and Y730F. AAV2(4pMut)dHS The AAV2...It is derived from AAV2 and has the following mutations: Y444F, Y500F, Y730F, T491V, R487G, R585S, and...from the AAV6 capsid and contains the following mutations: Y705F, Y731F and T492V. AAV44.9 The AAV44.9 serotype...Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F...Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose...

...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...increases specificity but is less efficient Each putative knockout allele must be experimentally verified...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...as there are for viral vectors — the only size limitation is what you can efficiently transfect. However...

...frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations in the DNA-binding...regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1 ...19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20..., often at one of six hotspot residues. These mutations can be inherited or arise through exposure to ... (e.g., HPV, the human papillomavirus). These mutations interfere with p53’s ability to activate transcription...Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function, expanding...;415(6867):45-53. PubMed PMID: 11780111 . p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein...

...The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol...by long terminal repeat (LTR) sequences, which facilitate integration of this portion into the host genome...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...(see Figure 2).  Figure 2: Schematic representation of integration process and episomal forms of...Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from aspartic acid...detecting vector particle components or performing quantitative PCR titration of vector DNA in samples harvested...Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of...

...protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - ...Capture methods such as immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate...which emit a specific wavelength of light after excitation by light of a specific wavelength. While more...interactions in a specific timeframe or context. Immunoprecipitation (IP) IP uses immobilized antibodies to isolate... on the same general strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate...isolating protein from serums. Chromatin immunoprecipitation (ChIP) This variation of IP uses antibodies...for individual assays. Clear cell debris by sedimentation. Proceed with IP using a primary antibody for...

..., the fluorescence lifetime (the time between excitation of the fluorophore and emission of a photon) ...-ShadowY , EGFP-ShadowY λ Dex : Donor maximum excitation wavelength (nm), QY D : Donor quantum yield, ...Overlap integral of the donor emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained...organism or cell type, relative position and orientation of the fluorophores through linkers, fluorescent...linkers of varying length Evers et al. (2006) Quantitative understanding of the energy transfer between...FRET efficiency standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative controls...

... purified through affinity chromatography. To facilitate the purification process, proteins are often ... reduces bacterial protein contamination and facilitates extraction. Browse our most popular plasmids ...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...fusing each of them to two FPs whose emission and excitation spectra overlap. That way if your proteins of...compatible vectors for bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...study the effects of protein expression in the stationary phase. In these cases, you may want to control...

...that can hold larger containers, but they can also rotate at much higher speeds and are used for more specialized...balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the...

...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...could allow you to conduct western blots or immunoprecipitations of your favorite gene even if you do...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag...methods. Delivery method Advantages Representative Empty Backbones AAV High transduction...expression, transgenics, and more. Element Details Representative Empty Backbones CRISPR Genome modification... Marker Typical Host Organism Representative Empty Backbones Neomycin (G418) ...Gateway vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene ...

...correcting a typo in the latest draft of your dissertation. Well, that will probably never happen. Yet ... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...for molecular tools. ZFs are not without their limitations, however. Not every nucleotide triplet has a ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition ...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit...in a C. elegans lab and have been struggling to mutate a specific gene or your anxiously investigating...

...contact with hazardous materials. A sink, eyewash station, safety shower, fire blanket, and extinguisher ... are located before you start. Use the eyewash station if unwanted or biohazardous materials are splashed...

...that the insert will be added in the correct orientation and prevents the vector from ligating to itself...indicates the various controls: Control Ligase Interpretation Uncut vector - Checks viability of competent...

...TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble TALEN constructs...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination...are designed to target and edit the epigenome of putative enhancers in mammalian cells. These plasmids allow...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...

...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...growth inhibitor 1 BDGI, OKL38 OSM oncostatin M MGC20461 OSMR oncostatin M receptor MGC150626, MGC150627,...have plasmids available for request. Antigen Presentation The cornerstone of the immune response is the...including dendritic cells and macrophages. Antigen presentation is mediated by the MHC class I and class II ...available for request. Antigen Processing and Presentation The cornerstone of the immune response is the...

...or not, without a guaranteed titer. Any titer expectations communicated by email will supersede the general...after you submit your request, so we can set expectations to the best of our knowledge from the start....not, with a longer turnaround time. Any timing expectations communicated by email will supersede the general...material received. Shipping While Addgene assists importation by including thorough shipping documents and ...taxes, and to complete any additional customs documentation that might be required by your country for the...

...loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

...continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent...adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc...This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease... cell division, all of which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

...autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with a working...thermometer. Light the flame at the plate pouring station and dilute your antibiotic into your ~60 ℃ molten...

...Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...

...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...shared experience for the research community and facilitates scientific collaborations between institutions...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

...for 3 h without stirring to allow full precipitation. Precipitation of the viruses can proceed overnight...