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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 191 results for: CO

Showing: 51 - 100 of 191 results

  1. CRISPR Plasmids - Plants

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    .... In this method, the major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and... research, which required protein compartments to co-localize multiple enzymes in a reaction pathway, ...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... firefly luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. ...catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with a gRNA... new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the limitations ...Firefly luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID..., and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested the ...

  4. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s... plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... DBDs (see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a ...your proteins are not post-translationally modified, co-expression of the enzyme responsible for the modification...

  9. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... protein (e.g., GFP or luciferase) that is either co-transfected with the test plasmid or transfected ...how much plasmid DNA to use for any individual or co- transfection, should be determined empirically.  ...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...1.1 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere...1.2 Affinity Frequently used for western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... tissue culture plate. Incubate cells at 37°C, 5% CO 2 overnight. Although cells should regularly be passaged...cells from the plate. i. Incubate cells at 37°C, 5% CO 2 for 12-15 hours. Day 3: j. In the morning, change... transfected cells. k. Incubate cells at 37°C, 5% CO 2 for 24 hours. Day 4: l. Harvest media from cells...antibiotics to the cells and incubate at 37°C, 5% CO 2 for 24 hours. Day 5: n. Harvest media from cells...target cells in ten 6 cm plates and grow at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be...1: a. Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should be approximately...your target cell line. d. Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature ...human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar...

  16. Brain Initiative Collection

    Type
    Collection
    ...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR...

  18. Rett Syndrome

    Type
    Collection
    ...ability to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests...D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and...

  19. General Transfection

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Gently aspirate media, add 10 mL fresh...

  22. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...enhances RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

  29. Lentivirus Production

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection...

  30. Antibody Production

    Type
    Collection
    ... recombinant antibody preps contain low levels of co-purified plasmid from transfection. This residual...

  32. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...cre recombinase (either from a mouse line or viral co-injection), the opsin is inverted into the correct...opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type specificity...

  33. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm ... in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm....

  34. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-...

  39. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... packaged in separate rAAV vectors which are then co-delivered to the host cells. The full-length transgene... sequence at its 5' end. When these viral vectors co-infect a cell, they form concatemers and splice together...both sequences present substantial sequence overlap. Co-expression induces homologous recombination and expression...

  40. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...2xNLS) fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort... in response to blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that...well as other photoactivatable proteins like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides...purify your POI, to perform pull down experiments like co-IPs and ChIP, and more - all without the need to ...specific genomic locus. When fused to the sgRNA and co-expressed with dCas9 – the dCas9, sgRNA/RNA cargo...

  48. TALEN Guide

    Type
    Collection
    ... ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger Consortium with Dr. Voytas...

  49. CRISPR Guide

    Type
    Guide
    ... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...
Showing: 51 - 100 of 191 results