...pollution Dr. Julio Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto...

... this blog post. I separate sgRNA design tools into database and de novo solutions. Database tools allow...community in order to succeed. With respect to de novo sgRNA design, I again separate it into two categories...single-target design”, “multi-target design”, “off-target aware”, “high mismatch limit”, “approximate design”, “... “empirical design”, “single-PAM design”, and “multi-PAM design”. Trends: By the end of 2014, tools began...with the post-design process such as primer and plasmid design. Examples:  “multiplex design”, “multi-method...determines good sgRNA design. Incidentally, the first software for sgRNA design (by Hsu et al., 2013) ...multi-method design”, and “single-method design”. Trends: The most common utility functions are batch design...

..., Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted evolution...

... Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins... I place the tag and how many do I need? When designing a plasmid containing a polyhistidine tag, you’...

...never been in contact with Aida Baida Gil of Coach De La Professional but the website looks interesting...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

...none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941...pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157...module vectors, including three designed for dicots and three designed for monocots. Using these gRNA ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you...Cas9 (or other CRISPR) system that the vector was designed to be used with, such as S. pyogenes, S. aureus...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit ...

....2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications. Plasmid...difference is virulence plasmids! This category includes genes that help bacteria invade hosts, including...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...found on an E. coli fertility plasmid that also encodes a toxin, CcdB, and the resistance to that toxin...

...doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C, Eason DE, Ross DL, Vachhani RV, Ming G-L, King JR, Song H,...Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool of choice...leave you with a few practical considerations: Design your gRNA to overlap the CpG of interest. Having..., too), but if this is not possible due to gRNA design restrictions, anything within 5 base pairs should...

...Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences. Sci...antibody function, improve antibody stability, and design tools that we haven’t even imagined yet. References...

...Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4...Specifically this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9...is an Assistant professor at the Biodesign Center for Molecular Design and Biomimetics and the School of...development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided by... toehold switches, which allow for the rational design of RNA sensors that can detect virtually any RNA...areas conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...

...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

... JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering...targeted organs or tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources on Addgene's CRISPR...Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome Editing by John Doench...

...https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam...Zimmermann et al., 2020). This selection platform was designed so that any standard lab can quickly select for...strongly to your target protein. The Seeger lab designed 3 different mRNA sybody libraries for use in the... randomized with a defined set of different trinucleotides (corresponding to different amino acids) in...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...

.... When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing our...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...

...392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk, M....of negative data.  Negative AAV data is highly desirable  In a survey we conducted to improve our Addgene...every researcher experiences and that it often provides incredibly valuable insight into one’s work. As... Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our vectors ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...the Data Hub. These minimum data standards are designed specifically to allow scientists to evaluate how...

... Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for...plasmids to produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep) and capsid (cap) genes...replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged into the...

...to sickle cell disease. Hoban MD, Orkin SH, Bauer DE. Blood. 2016 Feb 18;127(7):839-48. PubMed PMID: 26758916... but without sustained transgene expression. To design a viral vector system that could do both, Addgene...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats... knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested... check out our lentiviral plasmid and biosafety guides before beginning your experiment. Let us know how...

...Pietro De Camilli 194005 VPS13D1-1576-(Y1253S) VPS13D GFP CMV Spinocerebellar Ataxia Pietro De Camilli...145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1...SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP... GFP, Flag CMV Parkinsonism, early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin 1-170 SYNJ1...SYNJ1 GFP, Flag CMV Parkinsonism, early-onset Pietro De Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's...inserted at position aa 1914 CMV Parkinsonism Pietro De Camilli 118791 pHBS1383 TDP43 RRM-GFP FYW-S TARDBP...delta1235-1748 VPS13C Halo CMV Parkinsonism Pietro De Camilli 187767 MAC_C_CSF1R CSF1R MAC Hereditary diffuse...

...adenoviral vector infected cells does not require de novo viral gene expression: Implications for gene...a physiological effect of SARS-CoV-2 toward destabilizing one’s natural immune response to Adenovirus ...viruses infecting humans (from seven species, designated as letters A–G) exist. The life cycle of AdVs...short-lived. It is worth noting that it is possible to design a vector that achieves persistent transgene expression...

...science.aaq0180  Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced ...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately, certain drawbacks... approximately 1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates... proof-of-concept split intein SpCas9 which had modest editing rates in HEK-293T cells when compared to...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split...

... out to me were: Decrease the Number of Postdocs De-incentivize going into PhD programs as the standard...environments. Slide from Paula Stephan’s Talk (All slides can be found here). Importantly, the panel offered... joining long programs if it is obvious that a desired career outcome is not easily achieved. Of note...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

... 26 recombinant antibodies (rAbs) either produced de novo using yeast display technology or produced from...response, tissue repair, and kidney development. Despite their ubiquity and significance, these receptors...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces...

...26224555. PubMed Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...your downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

...Brugman, M. H., Pike-Overzet, K., Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill,...been a staple in molecular biology for over three decades. Widely used across various research applications...and human papillomavirus (HPV), to name a few (Desfarges & Ciuffi, 2012). One family of viruses, retroviridae... an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically...lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

...University of Oviedo, the European University Miguel de Cervantes, the University of Navarra, and the University...together with the universities, a bright future. IMP provides matched one-on-one international mentorship, with...recommended, a mentor with international experience provides his/her mentees with a broader, first-hand vision...

...applications. LuxSit-i : An artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase...to that of the fluorophore. Conformationally destabilized Firefly luciferase mutants : A set of three ...three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves ...dimerization , endosomal escape of cell-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of 5' promoter...service icon . ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Do you have suggestions...

...X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014. Genome-Scale CRISPR-Cas9...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows... store a record of invading phages but also to destroy the phages upon re-exposure. More specifically,...complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...very low off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR...

..., Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science . 2014 Jan 3;343(6166...oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed based on...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...digested using BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into...vector before the sgRNA scaffold. The oligos are designed based on the target site sequence (20bp) and need...using SAM: Addgene plasmids #61422 - #61424 were designed for transient transfection : 61422 : Expresses... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...

... Brewer, M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...will vary depending on your specific experimental design. Like selecting an antibody, some of the requirements...

...2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. ...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...promoters can be used in the repressible manner described above, as well as the inducible manner detailed...30 years since the original Tet-Off system was described by Gossen and Bujard, both the promoter and transactivator...cells, and can be used in the repressible manner described above, as well as the inducible manner detailed...tissue- or developmental stage-specific promoter and design a UAS reporter construct, the reporter will only...simple answer is that sometimes it is necessary or desirable to control or look at multiple genes at the same...

...doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...HDR event can’t occur. Lastly, depending on its design, donor A may be capable of expressing a truncated...

...oncogenic silencing in vivo by programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34...which uses light to regulate transcription, also includes light-regulated histone methyltransferases and...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9 to a transcriptional...epigenetic modifiers? As with any experiment, your desired outcome will determine the tool that you should...

...Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4...also be used to design primers to read states by qPCR or Sanger sequencing as described in the Material...hammerhead ribozyme parts are described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register...machine design space is highly degenerate and often times there are multiple register designs that may...recognition sites from the register. If the RSM is designed in such a way that each possible order of inputs...segments in each state of a register enables the design of primer pairs that PCR amplify in some states...registers within a sample using a metric that we describe in the Materials and Methods section of our paper...

...bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector...on channelrhodopsins – Chronos & Chrimson Through de novo sequencing of 127 algal transcriptomes, as well...components into a destination vector in a predefined order. Finally, the destination vectors are used ...engineering approach, they designed Opto-RTKs, which activate signaling cascades on exposure to low-intensity...years of distributing his Designer Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids ...minimizing confounding secondary effects? An approach described as “knocksideways” (a British idiom for “taking...recombination elements such as FRT or Lox sites, and provides a modular system for easier exchange and re-use...

... cell reprogramming factors and wait for cells to de-differentiate. However it may be difficult to decide...blog post Delivery Methods for Generating iPSCs provides an overview of several different reprogramming...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...

...Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322-derived multicopy plasmids harboring large...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric...copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization...Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production...

...policies that “muzzle” government scientists to the de-funding of crucial environmental research programs...and minority communities, where these issues hit hardest. According to the Guttmacher Institute, most women...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed... an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...science belongs to the taxpayers who fund it, and we deserve to know the truth about our world. Jonathan Aguilar...

...https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis... DNA double-strand breaks up to 54 bp from the desired insertion site result in efficient editing. Repair...available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...whether it be by improved gRNA or repair template design, delivery, screening, and more. Here are some highlights...highlights from the past few years:  Repair template design The Meyer lab meticulously characterized several...of editing. First, they devised guidelines for designing single-stranded repair templates (Farboud et al...Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...

... and B.Z. Stanger, Adult cell plasticity in vivo: de-differentiation and transdifferentiation are back...

... cell reprogramming factors and wait for cells to de-differentiate. However it’s difficult, especially... with peptides that help mediate their transduction, such as the polyarginine peptide described in Zhou...reprogrammer, to decide which method to use. This post provides a brief overview of reprogramming methods with... Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...

...Off-Spotter: tool for CRISPR/Cas design Program for designing optimal gRNAs. Provides feedback on number of potential...the Chan Zuckerberg Biohub . gRNA Design Deep learning models to design gRNAs and predict their efficiency... a new window) : a webtool that provides models for SpCas9 gRNA design. DeepBE (Link opens in a new window...window) : a webtool that provides models for base editor (BE) gRNA design. DeepPrime (Link opens in a ...window) : a webtool that provides models for prime editor (PE) pegRNA design. PMID 37119812 (Link opens...purpose of designing and evaluating experiments. Green Listed - a CRISPR Screen Tool Design gRNAs for ...Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT) gRNA design tool with extensive selection of eukaryotic...

...Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate ...Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru and Chad...

...more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you ...sequences it is time to design your gRNA oligos and clone these oligos into your desired vector. In many cases... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...Select Genetic Manipulation Select Expression System Design gRNA Publications Get Started CRISPR is a powerful...available for your particular experiment. Select Your Desired Genetic Manipulation Different genetic manipulations...mammalian cell lines Select Your Target Sequence and Design Your gRNA Once you have selected your CRISPR components..., you are ready to select a target sequence and design your gRNA. Know your cell line/organism and genomic...

...more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs Should...template vector design guidelines? A few notes below are considerations for designing HR donor. Generally...usually pick 3-6 guides around the region to find the most efficient guide (most guides would work, but... this ‘split RNA’ design is not as efficient in genome editing as the second design (in pX330 / PX335 ...comparable efficiency to the optimized chimeric design but with better accuracy (in other words, lower...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...published with thorough step-wise instructions on designing and using Cas9-based genome engineering tools ...

...CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO Zeo DEST - Tet-inducible...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His...Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds an N-terminal myristoylation...marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral Gateway destination vector, expression, CMV/...pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ...expression pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene ...needed for replication in the host cell and are designed for a user to insert a gene of interest. They ...

... pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are sensitive... or organisms for long-term imaging and can be designed to specifically localize to cellular compartments...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence ...environmental conditions. With proper experimental design, biosensors can enable investigation of a signaling...with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...

...contains many plasmid tools that were specifically designed for use in plants. These resources include tools... to fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general... Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens in a new window) for Targeted... Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones...Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding...Caixia Gao. Cloning The following collections are designed for creating plant expression vectors: A modular...MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons for chloroplast transformation...

...the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of...characterized which allow researchers to express desired constructs in precise cell types and/or developmental...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...

...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in C. elegans... CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian...Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast...Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use in Xenopus . ...Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to.... Zebrafish CRISPR plasmids designed for use in zebrafish....

...Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for proteome-...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...synthetic human phosphoproteome. The iSPI resource subdivides the 110,139 human phosphoserine site library ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable, proteome-scale...in a new window) rEcoli XpS and a new pSerOTS described in: System-wide optimization of an orthogonal ...