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We narrowed to 232 results for: SPL;

Showing: 51 - 100 of 232 results

  1. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... a wide audience. The dashboard that I made to display the data in the CSV file is open for anyone to ...Addgene Requests Dashboard   The dashboard will display the following items (Fig. 1): A row with four... the CSV is shown (figure 2) and the dashboard displays the data in a standardized way. Since Addgene ...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will...scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method for...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...talking to themselves as they compare the features displayed on the model with their mental model of the protein...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...target DNA strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian ...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...each sample. Successfully analyzed samples will display the following parameters: Sample - The unique ...analysis can be sorted by any of the parameters displayed in the summary table. You can also use your browser..., you’ll find an Indel plot (left panel) which displays the inferred distribution of indel sizes in the...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...sequence NGG compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may... AAV delivery. NmCas9, another small ortholog, displays lower off-target editing than wild-type SpCas9...rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 (Kim et al...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed...References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...abundant and ethanol concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong...complexity of the system, researchers have used split GAL4 architecture or combined the system with Flp...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  14. Brain Initiative Collection

    Type
    Collection
    ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-...

  15. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...negative effect on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence...cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions with multiple other ...

  16. Science as Art

    Type
    Blog Post
    ...Jurassic Technology in LA, where it is currently on display.   Many universities are now following the trend...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this...

  19. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...small (Rep52 and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of ...often driven by the AAV p40 promoter. Alternative splicing and differing translational start sites generate...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  21. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook from...similar apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega...

  23. RNA Extraction Without A Kit

    Type
    Blog Post
    ...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...

  25. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...

  26. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

  27. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

  28. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

  30. Pipetting Protocol

    Type
    Protocol
    ... expel liquid into the pipette tip, the volume display which shows the set volume for the the pipette,...section will help you understand how to read each display to dispense the correct amount of liquid reagent...), while 0.5 µL would read as 050. Each volume display on these pipettes will also have small tick marks...you will be rotating dials behind the numerical display or the top of the plunger of the pipette. Select...

  31. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

  32. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from...is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues...

  33. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...their characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9...Cpf1 candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9...

  35. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve..., neither of these can substitute for a liver transplant. My lab will combine bioengineered scaffolds ...

  36. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...light-sensitive systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable...., Wang, B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent...

  37. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients... recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala...

  38. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to...015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and...

  39. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...iATPSnFR1.0 and iATPSnFR1.1 (cytoplasmic, membrane-displayed, expression in neurons, astrocytes) A genetically... 1720648115. Katalin Torok Glutamate Membrane displayed variants of fluorescent glutatmate sensors (iGluSnFR1...

  40. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical characteristics...

  41. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

  42. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical model...

  43. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

  44. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

  45. Immunology Research Plasmids and Resources

    Type
    Collection
    ... regulatory factor X-associated protein - SHFM1 split hand/foot malformation (ectrodactyly) type 1 DSS1...oncogene homolog A (avian) MGC131774, NFKB3, p65 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...of sevenless homolog 2 (Drosophila) FLJ25596 SYK spleen tyrosine kinase DKFZp313N1010, FLJ25043, FLJ37489...Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies that bind...

  46. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

  47. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

  48. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

  49. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...
Showing: 51 - 100 of 232 results