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Showing: 51 - 100 of 156 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin...sensor for neuroscientists is CaMPARI. This sensor switches from green fluorescence to a red fluorescent state...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Photoactivatable / Photoconvertible: These proteins can switch their color when activated by a specific excitation...the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers ...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back to red at 12...N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...important ease of use is for them (if they are to switch from paper to digital for example): “… 99% of respondents...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...from this kit include the production of a suicide switch in cyanobacteria, synthetic inverters in E. coli...biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis ...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...importantly, you really are selling yourself. A sales pitch that requires reading two pages or even a paragraph...paragraph to get to the punch line is a terrible sales pitch. State in the first sentence or two what your research...

  11. Retrograde AAV viral preps

    Type
    Collection
    ...category by using the buttons. Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors...EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...researchers have begun to use anti-CRISPRs as off-switches for CRISPR genomic editing. Genome Engineering...KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9. Cell . 167(7):1829-1838. PMID...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    ...relatively cheap and easy to source, making it easy to switch from one readout method to another or change primary...’ll have to use different concentrations if you switch from one to the other.  Type of gel The type of...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    ...or off, literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available...

  23. Editor's Choice, August 2016

    Type
    Blog Post
    ...Management Software for Biology Adapting Toehold Switches to Detect Zika Virus As always, if you have any...

  25. Synthetic Biology - Overview

    Type
    Collection
    ...Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent...

  31. Hot Plasmids - October 2022

    Type
    Blog Post
    ...articles. Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps...

  33. Antibody Plasmid Collection

    Type
    Collection
    ... secrete antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional...

  34. mTOR Pathway

    Type
    Collection
    ...nutritional state of the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1...

  50. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ ...
Showing: 51 - 100 of 156 results