We narrowed to 835 results for: ANG

... producing antibodies, can change the constant region, and therefore change the isotype of antibodies ...variable regions, shown in light blue and light orange, that bind to a specific antigen, triggering an...constant region, and is shown in dark blue and dark orange. The constant region is a stable part of the antibody... There are several alternatives to antibodies, ranging from antibody fragments (shown in Figure 2) to ...expensive than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing... assay run. The standard curve should contain a range inclusive of all expected protein concentrates in...to distinguish cell types. Multiplex assays can range from two antibodies to upwards of twenty. Laser ...

...mutations, base changes, and large insertions or deletions. For more information on the wide range of CRISPR...transcriptional activators that need to be within a given range of the transcription start site in order to function...Plasmid delivery is versatile thanks to the wide range of options for the Cas enzyme, promoters, and selection...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...heterozygous edits High sensitivity (good for rare edits) Sanger sequencing Knockouts Prime and base edits Good ...interference Needs a good antibody References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...

... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...Cre, or FRT for Flp). The nature of the DNA rearrangement after recombination (inversion, deletion, or ...Strategies Depending on how the target sites are arranged, recombinases can be used for more than inversion...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts Oligodendrocyte Precursor Cells Lentiviral...ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human... Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion...Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis...

...specialized Promega technologies that support a range of applications. The technology includes NanoLuc...enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are ideal for... a fusion protein in your hands, thanks to interchangeable ligands. HaloTags form a covalent bond with...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous ...

...targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove.... A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...available from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature...

...accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual ...

...Link opens in a new window) . Zhang, J., Chen, B., Gan, C., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive...in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin RNA ...

...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from the Michael Bassik lab , in...

... (Addgene #16403) plasmid with Gateway cassette Zhang 50958 ShuttleNSE For production of viruses containing...viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP For production of viruses containing...production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids Browse commonly...

...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps ... The PCR product is purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence...

...EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal adhesions Paxillin PSmOrange Vladislav Verkhusha 36991 Desmoglein2...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...Clathrin-coated vesicles Clathrin light chain EYFP Xiaowei Zhuang 164462 Clathrin-mGreenLantern Clathrin-coated vesicles...

...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

...resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating Gene Expression...none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) ...marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI...

...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases... of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements... memory loss, deficits in decision-making, and language abilities. The disease can be classified as early-onset...

...under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector, expressing...reduced background expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org...transcriptional activators: novel mutations yield expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (...

...Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman Konrad Hochedlinger...Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family...

...Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson...Yellow Plasmid Description Industry Return to top Orange Plasmid Description Industry Return to top Red ...

...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously...