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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 838 results for: ANG

Showing: 21 - 40 of 838 results

  1. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest fused ... GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest fused...to measure protein interactions or conformation changes, as well as FRET standards. Plasmid...intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and acceptor are...fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used ...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase...transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of ...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK...EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return to Top Reporter Constructs Already know what...brighter signal and emission in the near-infrared range, ideal for in vivo imaging. teLuc : Red-shifted ... on protein-protein interactions, drug-induced changes, and more. NanoBit (Link opens in a new window)...

  3. Antibody Guide

    Type
    Collection
    ... producing antibodies, can change the constant region, and therefore change the isotype of antibodies ...variable regions, shown in light blue and light orange, that bind to a specific antigen, triggering an...constant region, and is shown in dark blue and dark orange. The constant region is a stable part of the antibody... There are several alternatives to antibodies, ranging from antibody fragments (shown in Figure 2) to ...expensive than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing... assay run. The standard curve should contain a range inclusive of all expected protein concentrates in...to distinguish cell types. Multiplex assays can range from two antibodies to upwards of twenty. Laser ...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...via use of CRISPR-Cas9. Wu Y, Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem...cells are used for DNA extraction, although a wide range of cell numbers is acceptable. The bulk sorted cells..., mini-prep each one, and subject each clone to Sanger sequencing to characterize each deletion allele...Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols . 2013. PubMed . Characterization...colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation...

  5. Plan Your Experiment

    Type
    Collection
    ...mutations, base changes, and large insertions or deletions. For more information on the wide range of CRISPR...transcriptional activators that need to be within a given range of the transcription start site in order to function...Plasmid delivery is versatile thanks to the wide range of options for the Cas enzyme, promoters, and selection...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...heterozygous edits High sensitivity (good for rare edits) Sanger sequencing Knockouts Prime and base edits Good ...interference Needs a good antibody References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...51295 pRGEB31 rice snoRNA U3 BsaI none S. pyogenes Yang 50579 pBUN6A11 OsU3 BsaI yes, activate S. pyogenes...pRGEB32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang 62201 pHSE401 AtU6-26 yes, cut S. pyogenes Hyg Chen...pRGE32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang 62202 pKSE401 AtU6-26 yes, cut S. pyogenes Kan Chen...50929 pRGE31 rice snoRNA U3 BsaI none S. pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. ...

  7. Ras Pathway

    Type
    Collection
    ... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...Cre, or FRT for Flp). The nature of the DNA rearrangement after recombination (inversion, deletion, or ...Strategies Depending on how the target sites are arranged, recombinases can be used for more than inversion...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  9. COVID-19 Resources

    Type
    Collection
    ...receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another key step is the priming of ...the links and references below: ACE2 - (Human Angiotensin Converting Enzyme 2) is the host cell receptor...nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural...design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol for using...Medicine The proximal origin of SARS-CoV-2 . BSG - Wang, K et al, 2020, BioRxiv SARS-CoV-2 invades host ...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts Oligodendrocyte Precursor Cells Lentiviral...ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human... Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion...Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis...

  11. Promega Plasmid Collection

    Type
    Collection
    ...specialized Promega technologies that support a range of applications. The technology includes NanoLuc...enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are ideal for... a fusion protein in your hands, thanks to interchangeable ligands. HaloTags form a covalent bond with...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous ...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal Adhesions paxillin PSmOrange Vladislav Verkhusha 79999 pZyxin-miRFP703...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari ...

  13. TALEN Guide

    Type
    Collection
    ...targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove.... A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...available from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature...

  14. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual ...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...Link opens in a new window) . Zhang, J., Chen, B., Gan, C., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive...in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin RNA ...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

  17. Adenovirus Plasmids

    Type
    Collection
    ...Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with almost...Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable...tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular inserts...

  18. Antibody Production

    Type
    Collection
    ...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps ... The PCR product is purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence...

  19. Microbiology Resources

    Type
    Collection
    ...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

  20. Plant Plasmids and Resources

    Type
    Collection
    ...window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes...genome editing in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (including...window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible innovation...
Showing: 21 - 40 of 838 results