...Learn more about Addgene's Beta Projects and how you can provide us with input and feedback on this service...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells...Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning grade DNA? At this...and will require separate shipping charges. Where can I find cloning resources or scientific support? Please...

...level. By controlling the number of mismatches we can obtain a range of expression levels. This collection...Detailed information about the repression system can be found in this article: Vigouroux, A., Oldewurtel...information about chromosomal integration of pLC97 can be found in this article: St-Pierre, F., Cui, L.,...the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array ...

... generate TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast...expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7...and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids and improve ...domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand annealing assay, which ...GoldenGate Charles Gersbach These new destination vectors can be used to create TALE-transcription factors and ...fused with mClover and mRuby2, respectively, and can be used as destination vectors for Voytas Golden ...using these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4)...

...separate) tdTomato 8 Scanziani Tools for Cell Ablation These AAV encode tools that can be used for cell ablation...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...AAV encode tools for affinity purification (which can be used for viral TRAP). ID Name Expression System...Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments, including...

...assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details... the REAL, REAL-Fast, and FLASH assembly methods can be found on the TALengineering website . All three...amino-terminus. The expression vectors listed below can be purchased together with the Joung Lab Individual...

...gene of interest. Co-expression of dCas9 and sgRNA can efficiently block transcription (in bacteria, ~300... the target loci. Thus, CRISPRi is a system that can regulate a genome instead of modifying a genome. ...vector with ampicillin resistance, wherein the N20 can be custom designed to target arbitrary sequences ...promoter controlled sgRNA cassette, wherein the GN19 can be custom designed to target sequences in the genome....044. PubMed PMID 23849981 . Individual plasmids can be ordered via the links below: ID Plasmid Description...

...lineage conversion can be faster, bypasses intermediate pluripotent stages, and can occur without cell...available from Addgene that can be used to create iPSCs or another cell type. You can also search the iPSC Neurodegenerative...Pluripotent stem cells, such as embryonic cells, can potentially give rise to any other cell type in the...stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell types, but are typically... cell use and has the major advantage that iPSCs can potentially be derived from patient-specific or disease-specific...the table below. Once iPSCs have been created they can be directly differentiated into specific somatic ...Disease Initiative (iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged...

...collection can be used for screening transcription factors for cellular programming or cancer drug resistance...library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor...isoform in pooled screens. This collection and library can be used for screening transcription factors for applications... applications such as cellular programming or cancer drug resistance. Protocols & References A transcription...

...in Japan; Identify candidate gRNA target sequences in an input sequence, which can be an accession number...mutagenesis The amplicon sequence expected after HDR can be provided as an optional input to assess HDR frequency...frequency. An sgRNA sequence (without PAM sequence) can be provided, to compare the predicted cleavage position...Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively selected sgRNAs...selection of eukaryotic pathogen genomes (200+) that can predict gRNA targets in gene families, HDR oligonucleotide...), and yeast (strain w303). CRISPR MultiTargeter Can be used to identify novel gRNA target sites in a ...input sequence, checks for off-target binding and can work for S. pyogenes, S. thermophilus or N. meningitidis...

...biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved...genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification...

...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which...For example, chromosome-specific repetitive loci can be efficiently visualized in living cells using a... close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that...

...an Order: After you have created an account, you can place orders through our website. Please note that...Recipient Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who ...institution. An authorized signatory is someone who can execute legal documents on behalf of the recipient...recipient institution. Once the MTA has been signed, it can be submitted to Addgene through your user account...collection Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...orders must be placed online through our website. We cannot accept orders by phone, fax, or email. Pricing ...

.... If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose a.... It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection... listed on the Function section pages above. You can also do a search of viral preps and filter by the...

...epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence methods can be used...systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR...

... single-strand DNA degradation. This degradation can continue for many kilobases in one or both directions...effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions... into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms...

... binds tightly to a specific antigen, scientists can use it to pull out a protein of choice from a mix...the R-mAbs are secreted into the culture media and can be collected for use. Other scientists may choose...small size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation...following table highlights plasmid collections that can be used to create and express plasmid-based antibodies...

..., and, when dysregulated, can enable cancer cell survival. Although cancers are partially defined by their... variants found across cancer types as part of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set... Special Collections Cancer Research Cancer Research...Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute... for cancer research, organized by signaling pathway. Special...Addgene has a large variety of resources for use in cancer signaling studies, including mutant, knockdown,...pathway, focusing on the main pathways involved in cancer development. These pathways are important to normal...

...protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

...remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality...expression in Escherichia coli bacteria. Researchers can use the collection as the basis of manufacturing ...choice and transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues... where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or...evolution. More information about the collection can be obtained at the Open Bioeconomy Lab's Open Enzyme...

...This serine phosphosite library can then be used to identify candidate human substrates of kinases of ...labs at Yale University changed the way researchers can explore important questions surrounding serine phosphorylation...quantitative assessments. The mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ...protein-protein interactions. The phosphosite DNA library can also be introduced into a second expression vector...mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell sorting...Screening kinases. The mode #1 phosphosite library can also be synthesized using supD tRNA ( 68307 ) to ...