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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,074 results for: CAN

Showing: 21 - 40 of 1074 results

  1. AAV Molecular Tools

    Type
    Collection
    ...separate) tdTomato 8 Scanziani Tools for Cell Ablation These AAV encode tools that can be used for cell ablation...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...AAV encode tools for affinity purification (which can be used for viral TRAP). ID Name Expression System...Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments, including...

  2. TALEN Expression Vectors

    Type
    Collection
    ...assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details... the REAL, REAL-Fast, and FLASH assembly methods can be found on the TALengineering website . All three...amino-terminus. The expression vectors listed below can be purchased together with the Joung Lab Individual...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...lineage conversion can be faster, bypasses intermediate pluripotent stages, and can occur without cell...available from Addgene that can be used to create iPSCs or another cell type. You can also search the iPSC Neurodegenerative...Pluripotent stem cells, such as embryonic cells, can potentially give rise to any other cell type in the...stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell types, but are typically... cell use and has the major advantage that iPSCs can potentially be derived from patient-specific or disease-specific...the table below. Once iPSCs have been created they can be directly differentiated into specific somatic ...Disease Initiative (iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged...

  4. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...collection can be used for screening transcription factors for cellular programming or cancer drug resistance...library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor...isoform in pooled screens. This collection and library can be used for screening transcription factors for applications... applications such as cellular programming or cancer drug resistance. Protocols & References A transcription...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved...genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification...

  6. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which...For example, chromosome-specific repetitive loci can be efficiently visualized in living cells using a... close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that...

  7. Distribution to Industry

    Type
    Collection
    ...an Order: After you have created an account, you can place orders through our website. Please note that...Recipient Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who ...institution. An authorized signatory is someone who can execute legal documents on behalf of the recipient...recipient institution. Once the MTA has been signed, it can be submitted to Addgene through your user account...collection Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...orders must be placed online through our website. We cannot accept orders by phone, fax, or email. Pricing ...

  8. AAV Viral Preps

    Type
    Collection
    .... If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose a.... It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection... listed on the Function section pages above. You can also do a search of viral preps and filter by the...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... single-strand DNA degradation. This degradation can continue for many kilobases in one or both directions...effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions... into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells...Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning grade DNA? At this...and will require separate shipping charges. Where can I find cloning resources or scientific support? Please...

  11. Antibody Plasmid Collection

    Type
    Collection
    ... binds tightly to a specific antigen, scientists can use it to pull out a protein of choice from a mix...the R-mAbs are secreted into the culture media and can be collected for use. Other scientists may choose...small size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation...following table highlights plasmid collections that can be used to create and express plasmid-based antibodies...

  12. Cancer Research Plasmids and Resources

    Type
    Collection
    ..., and, when dysregulated, can enable cancer cell survival. Although cancers are partially defined by their... variants found across cancer types as part of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set... Special Collections Cancer Research Cancer Research...Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute... for cancer research, organized by signaling pathway. Special...Addgene has a large variety of resources for use in cancer signaling studies, including mutant, knockdown,...pathway, focusing on the main pathways involved in cancer development. These pathways are important to normal...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ...protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  14. Open Enzyme Collection

    Type
    Collection
    ...remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality...expression in Escherichia coli bacteria. Researchers can use the collection as the basis of manufacturing ...choice and transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues... where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or...evolution. More information about the collection can be obtained at the Open Bioeconomy Lab's Open Enzyme...

  15. CRISPR Plasmids and Resources

    Type
    Collection
    ...epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence methods can be used...systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...This serine phosphosite library can then be used to identify candidate human substrates of kinases of ...labs at Yale University changed the way researchers can explore important questions surrounding serine phosphorylation...quantitative assessments. The mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ...protein-protein interactions. The phosphosite DNA library can also be introduced into a second expression vector...mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell sorting...Screening kinases. The mode #1 phosphosite library can also be synthesized using supD tRNA ( 68307 ) to ...

  17. Plant Plasmids and Resources

    Type
    Collection
    ... that can be used as empty backbones for your gene of interest for expression in plants. You can search... Transcription Factors The following collections can be used for studying gene expression in plants: Maize...luminescent proteins for expression in plants. You can search the table for features you are looking for...

  18. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've...this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety...reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle...uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms blue colonies...

  19. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to...plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-... Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger expression of the downstream gene...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest...description, meaning they contain a TRE or tet O and can serve as either Tet-Off or Tet-On systems, depending...vectors. Stable cell lines or transgenic animals can be made to achieve greater control over expression...example, only a relatively low level of doxycycline can access the brain so a highly sensitive transactivator...profiles for dox and other tetracycline derivatives can be found in Krueger et al., 2004. Some cell culture...may contain tetracycline or its derivatives which can result in unintended induction. Check the manufacturer...
Showing: 21 - 40 of 1074 results