Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 430 results for: ERG

Showing: 21 - 40 of 430 results

  1. mTOR Pathway

    Type
    Collection
    ...synthesis, and increases energy production through glycolysis to fuel these energy-intensive processes. mTORC1...signals, including amino acids, stress, oxygen, energy levels, and growth factors. mTORC1 integrates these...including an abundant supply of macromolecules and energy, as well as the presence of both pro-proliferative...machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...diseases was curated from several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to ...eIF4GI (1-1599) EIF4G1 HA CMV Parkinson's Nahum Sonenberg 45875 pEGFP-parkin WT PRKN GFP CMV Parkinson's...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Background Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred...fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor...pairs in FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy...

  4. Lentivirus Plasmids

    Type
    Collection
    ...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag... with 2nd and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for...resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...-CRISPR/Cas9 strategy for gene editing across divergent rodent species. Boender et al. Sci Adv. 2023 Jun... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat Methods. 2020...30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout . Maimaitili...resource was created with support from the Chan Zuckerberg Initiative (Link opens in a new window) ....

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced...PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein... editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . ...aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  8. Antibody Production

    Type
    Collection
    ...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted...Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The...distributed. Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (...Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range..., Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a... Mouse IgG2a 220412 Beta-2 adrenergic receptor [N430/30R] Beta-2 adrenergic receptor Mouse Mouse IgG2a...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...scFv] N324/2 Rem2 Rat Mouse 219492 Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv] N330A/80 Alpha-...

  11. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA...NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...dJ579N16.4 ADRB1 adrenergic, beta-1-, receptor ADRB1R, B1AR, BETA1AR, RHR ADRB2 adrenergic, beta-2-, receptor...CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 glycoprotein hormone ...activates the T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-...LHRHR, LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR ...

  14. Cre-lox system

    Type
    Collection
    ...-EBD - Estradiol-dependent control Gal1 Yeast Gartenberg 50797 pUAS-Cre Cre UAS/Hsp70 Mammalian Cepko ...plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can be useful...Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg N, Hamilton D. 1981. Bacteriophage P1 Site-Specific...

  15. COVID-19 Resources

    Type
    Collection
    ...sequences are now available and more are currently undergoing quality control analysis in our lab and will ... window) AapCas12b plasmid from the Abudayyeh-Gootenberg lab. A one-enzyme RT-qPCR assay for SARS-CoV-...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...3rd 10 Varies Berger Lab Human Paralog Knockout Library (pgPEN) 171172 Knockout Human Berger 3rd 8-16 33,170...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...antibodies. Detergents  Another common family of permeabilization reagents are detergents, such as Triton...these detergents avoid some of the negative effects caused by organic solvents. However, detergents can ...for what reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the... X-100 and Tween-20 are examples of non-ionic detergents that interact with lipids and proteins in membranes...protein. B) Permeabilizing with the selective detergent, saponin, keeps membrane-associated proteins in... epitopes. C) Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA...with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et...Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase...the CRISPR-transposon systems developed by the Sternberg lab derives from Vibrio cholerae (V. cholerae ...CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT, three separate...Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He ....     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

  20. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...
Showing: 21 - 40 of 430 results